Lactate dehydrogenase (LDH) derived from rabbit muscle enhanced IgM production by human-human hybridoma HB4C5 cells 12.4-fold at 320 mug/ml under serum-free conditions. LDHs from pig muscle and pig heart also accelerated IgM production 8.4- and 6.4-fold, respectively. The immunoglobulin production stimulating activity of LDH was not accompanied by activation of cell proliferation. LDH from rabb...

BACKGROUND : Serum and sputum eosinophil cationic protein (ECP) levels are correlated with asthma disease severity. OBJECTIVE : To establish a diagnostic accuracy of ECP and lactate dehydrogenase (LDH) in serum (indirectly) and sputum (directly) as inflammatory markers in asthma. PATIENTS AND METHODS : In a cross sectional study, 76 asthmatic patients with exacerbation were enrolled in the ...

A unique electrophoretic form of lactate dehydrogenase (LDH-Z), formerly observed in a choriocarcinoma cell line (JEG 3) and first-trimester placenta, has been shown to be the same as that produced in hydatidiform mole and term placenta. We have also observed LDH-Z in second-trimester placenta, cho riocarcinoma metastasized to the liver, and five of five addi tional independently derived (from ...

application of nanotechnology in medicinal and biological fields has attracted a great interest in the recent yeras. in this paper the cell membrane leakage induced by iron nanoparticles (fe-np) against pc12 cell line which is known as a model of nervous system cell line was investigated by the lactate dehydrogenase (ldh) test. therefore, pc12 cells were incubated with different concentration o...

L-Lactate dehydrogenase (L-LDH, E.C. 1.1.1.27) is encoded by two or three loci in all vertebrates examined, with the exception of lampreys, which have a single LDH locus. Biochemical characterizations of LDH proteins have suggested that a gene duplication early in vertebrate evolution gave rise to Ldh-A and Ldh-B and that an additional locus, Ldh-C arose in a number of lineages more recently. A...

Fluorescence spectroscopy was used to study some aspects of thermal denaturation of lactate dehydrogenase (LDH) from rabbit muscle, in the absence and presence of urea and NaCl. Thermal denaturation of LDH seems to be one step and a slightly reversible process. The solution pH has an important influence on the LDH thermal denaturation. The interaction of LDH enzyme with NADH ligand was also inv...

In many tumor types serum lactate dehydrogenase (LDH) levels is an indirect marker of tumor hypoxia, neo-angiogenesis and worse prognosis. However data about hepatocellular carcinoma (HCC) are lacking in the clinical setting of patients undergoing transarterial-chemoembolization (TACE) in whom hypoxia and neo-angiogenesis may represent a molecular key to treatment failure. Aim of our analysis w...

Lactate dehydrogenase 5 (LDH-5) is one of the major isoenzymes catalyzing the biochemical process of pyruvate to lactate. The purpose of this study was to investigate the expression of serum LDH-5 and test whether this enzyme is regulated by tumor hypoxia and represents a prognostic marker in patients with Non-Hodgkin's lymphoma (NHL). In this study, LDH-5 levels were detected using agarose gel...

Differential expression of isozymes enables fish to tolerate temperature fluctuations in their environment. The present study explores the modulation lactate dehydrogenase (LDH) and cytoplasmic malate (sMDH) isozyme heart, muscle, brain, liver, gill, kidney juvenile Horabagrus brachysoma after 30 days acclimation at 26, 31, 33, 36°C. LDH sMDH zymography were performed using native polyacrylamid...