Hemoglobin A2 (Hb A2) and hemoglobin F (Hb F) are important analytes in the diagnosis and follow up of Hb diseases. We evaluated a new capillary zone electrophoresis (CZE) kit for Hb A2 and Hb F measurements. The imprecision ranged from 3% to 6% for Hb A2 and Hb F at physiological and pathological concentrations. The method compared well with cation-exchange HPLC for Hb A2 and Hb F and with ani...

A new turbidimetric method for the direct measurement of the solubility of oxy- and deoxyhemoglobins (Hb) in concentrated phosphate buffer has been established. The principle of the method is the formation of a homogeneous emulsion when hemoglobin is introduced in concentrated phosphate buffer. The solubility of the oxy and deoxy forms of Hb A, Hb S, Hb C, Hb F, and Hb CHarlem (beta 6Glu leads ...

Background: Hemoglobin (Hb) and hematocrit (Hct) have been used routinely in the diagnosis of neonatal anemia and polycythemia. Objective: To measure the cord blood Hb and Hct and to determine their association with maternal Hb and Hct. Methods: 170 healthy neonates born through normal vaginal delivery with gestation age of 37-42 wks and birth weight of 2.5-4 kg, and their mothers were included...

BACKGROUND Hemoglobin variants (Hb(VAR)) are not uncommon in the Korean population, with Hb G-Coushatta and Hb Queens being the 2 most common Hb(VAR). Hb G-Coushatta is also the most common Hb(VAR) in Chinese people from the Silk Road region, as well as in some North American Indian tribes. However, data are scarce on the effect of these Hb(VAR) on the different methods used for analyzing HbA(1...

A 52-year-old woman with a medical history of hepatitis B, hyperlipidemia, hypertension, anemia, and depression presented to the internal medicine clinic for a routine visit. Laboratory tests 3 months previously had revealed an impaired fasting glucose concentration of 5.9 mmol/L (106 mg/dL) [reference interval, 3.9 –5.6 mmol/L (70 –100 mg/dL)]. Therefore, a hemoglobin (Hb) A1c analysis was per...

A diluted solution of a mixture of Hb S and Hb A in concentrated phosphate buffer was found to aggregate with a clear demonstration of delay time. The mechanism of the participation of Hb A in the aggregation of deoxy Hb S in concentrated phosphate buffer has been investigated. Both the delay time and the aggregation time in the polymerization reaction of mixtures of Hb A and Hb S were prolonge...

The functional properties of four glycosylated minor components of human hemolysate have been examined. In 0.1 M potassium phosphate buffer, pH 7 and 2O”C, the p50 values of Hb Al,,, Hb Alaz, Hb Al,, and Hb A, were 14,9.8,6.3, and 7.4 mm Hg, respectively, compared to 7.8 mm Hg for the major component, Hb Ao. The A log p&A pH (pH 7 to pH 8) was -0.5 for Hb A1 .1, Hb Alaz, and Hb A1, as compared ...