The C-terminal domains of Dnmt3a and Dnmt3L form elongated heterotetramers (3L-3a-3a-3L). Analytical ultracentrifugation confirmed the Dnmt3a-C/3L-C complex exists as a 2:2 heterotetramer in solution. The 3a-3a interface is the DNA-binding site, while both interfaces are essential for AdoMet binding and catalytic activity. Hairpin bisulfite analysis shows correlated methylation of two CG sites ...

The transfer of agronomically useful genes from wild wheat species into cultivated wheat is one of the most effective approaches to improvement of wheat varieties. To evaluate the transfer of genes from Dasypyrum villosum into Triticum aestivum, wheat quality and disease resistance was evaluated in two new translocation lines, T1DL•1V#3S and T1DS•1V#3L. We examined the levels of stripe rust res...

In a paper published in the International Journal of Geriatric Psychiatry (Sanchez-Arenas et al. 2014), Sanchez-Arenas et al. concern themselves with the reliability of the EQ-5D-3L, which they refer to solely as the EQ-5D. They investigate both the reliability of the five single dimensions and the reliability of the whole instrument. Sanchez-Arenas et al. report that they have determined the r...

Much remains to be discovered about the etiology of heart valve disease and the molecular level mechanisms that drive it. The MAPK/ERK pathway influences calcification in many cell types and has been linked to the expression of a contractile phenotype in valvular interstitial cells (VICs). However, a direct correlation between MAPK/ERK pathway activity and VIC calcification has not been previou...

Department of Pharmacy Practice, Faculty of Pharmacy, Monash University, Melbourne, VIC. Johnson George, MPharm, PhD Scholar; David C M Kong, MPharm PhD, Lecturer; and Senior Pharmacist, The Alfred, Melbourne, VIC; Kay Stewart, BPharm(Hons), PhD, Senior Lecturer. Ambulatory and Community Services, The Alfred, Melbourne, VIC. Lisa L Ioannides-Demos, BPharm, PhD, Senior Research Scientist; and Re...