We performed spoligotyping and 12-mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTRs) typing to characterise Mycobacterium bovis isolates collected from tissue samples of bovines with lesions suggestive for tuberculosis during slaughter inspection procedures in abattoirs in Brazil. High-quality genotypes were obtained with both procedures for 61 isolates that ...

In many countries, Mycobacterium tuberculosis isolates are routinely subjected to variable-number tandem-repeat (VNTR) typing to investigate M. tuberculosis transmission. Unexpectedly, cross-border clusters were identified among African refugees in the Netherlands and Denmark, although transmission in those countries was unlikely. Whole-genome sequencing (WGS) was applied to analyze transmissio...

Estimates of genetic diversity within, and of variation among, semi-isolated populations of the common toad (Bufo bufo) were derived and compared across four classes of nuclear genetic markers, including allozymes, microsatellites, and single and multilocus minisatellites. Estimates of multilocus heterozygosity and the number of alleles per locus derived from allozymes were lower than for each ...

The contribution of polymorphic markers rs2234663 (VNTR) and rs419598 (c.2008T>C) the IL1RN gene to predisposition development arterial hypertension among population Karelia was studied. occurrence alleles genotypes for these almost same in group healthy people patients with (χ2 = 0.178, p 0.67; χ2 0.540, 0.76; 0.01, 0.93, 1.68, 0.43, respectively rs419598). level IL-1β IL-1α plasma did not ...

Variable number of tandem repeats (VNTR) polymorphism in the interleukin 4 (IL-4) gene has been associated with end-stage renal disease (ESRD) subjects in many different populations, although with conflicting results. We determined the 70 bp of VNTR polymorphism at intron 3 of the IL-4 gene in Malaysian ESRD subjects. Buccal cells were collected from 160 case and 160 control subjects; genomic D...

For people working in dental prosthesis laboratories (DPL), metal alloys and methylmethacrylate-based monomers polymers used the laboratory can pose occupational risks. These risks cause health problems toxic effects on human health. This study purposes of researching exposure because manufacturing operations variable number tandem repeat (VNTR) polymorphisms IL-1Ra IL-4 genes. 29 DPL workers, ...

Phenylketonuria (PKU) is one of the most common metabolic inborn diseases caused by mutations in the phenylalanine hydroxylase (PAH) gene. This gene is linked to a variable number of tandem repeats (VNTR) region which is a polymorphic marker that facilitates the implementation of prenatal diagnosis and carrier screening. In this study, VNTR with 13 repeats that has not been reported previously ...

Apolipoprotein B gene 3' variable number tandem repeat (VNTR) and related regions were amplified by PCR and analyzed by agarose gel electrophoresis. Eighteen VNTR alleles (VNTR25, 26, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 58, 60) and 45 genotypes were observed in 477 Taiwanese subjects. The VNTR35 allele and genotype VNTR35/35 were observed most frequently in this population. ...

BACKGROUND AND OBJECTIVES Molecular typing methods are important and useful tools to assess the transmission, diversity of strains and differentiation between new infections and relapses which can effectively help in controlling infections. The aim of this study was to evaluate the molecular typing methods which have been used in Iran. By evaluating the results and discriminatory power of each ...