BACKGROUND AND AIM OF THE WORK In recent years, the diagnosis of toxoplasmosis has been improved by Real-time PCR assays. In this study we compared the performances of two Real-time PCRs (FRET and TaqMan protocols) already described in the literature, and one nested-PCR, currently used in our laboratory for the molecular diagnosis of toxoplasmosis. METHODS We evaluated the sensitivity and the...

Diagnosis of pulmonary tuberculosis (TB) is difficult in cases with an unusual presentation and often requires a lung biopsy. The goal of this study was to determine the clinical usefulness of nested PCR on lung tissue for the diagnosis of pulmonary TB. Clinical and laboratory data were reviewed from patients who underwent diagnostic lung biopsies, followed by nested TB PCR on formalin-fixed pa...

conclusions in this study, high prevalence of 91.7% hcmv gb1 genotype was predominant among hcmv-positive uc patients, which suggests that there might be an association between hcmv gb genotype 1 and uc disease. results among 98 patients with uc, only 12 (12.2%) patients were positive for hcmv genome, while the hcmv genome was not detected in any of the controls. (p = 0.002). the distribution o...

conclusions in molecular epidemiology studies, nested pcr has higher sensitivity than pcr. results of this study support the colonization of p. jiroveci in patients receiving lung transplantation. patients who are carriers of p. jiroveci are at a higher risk of p. jiroveci pneumonia. results the genome of p. jiroveci was revealed by a 346 bp pcr product in the initial amplification and a 120 bp...

background: q fever is an important widespread reemerging zoonosis. the presence of coxiella burnetii in 100 tick-infested dogs was assessed in this study. methods: the blood samples from 100 referred dogs were acquired and evaluated by nested-pcr. results: c. burnetii was detected in 11 out of 100 (11%) blood samples. most of the positive dogs were kept outdoor and fed on raw diet. based on ou...

background: species of microsporidia have been known as opportunistic obli-gate intracellular parasites particularly in immunocompromised patients. enterocyto-zoon bieneusi is one of most prevalent intestinal microsporida parasites in hiv + /aids patients. in this study, intestinal microsporidia infection was deter-mined in hiv + /aids patients using microscopic and molecular methods. methods: ...

background: the objective of the study was to evaluate the presence of neospora caninum organisms in the brain of aborted fetuses and placentas of full-term calves born of seropositive cows. methods: during 2006-2007, 12 brains of aborted calves from neospora seropositive cattle and 7 pla­centas from seropositive dams giving birth to full-term calves, from four dairy cattle farms located around...