We compare the effectiveness of solving Dirichlet–Neumann problems via the Craig–Sulem (CS) expansion, the Ablowitz-Fokas-Musslimani (AFM) implicit formulation, the dual AFM formulation (AFM∗), a boundary integral collocation method (BIM), and the transformed field expansion (TFE) method. The first three methods involve highly ill-conditioned intermediate calculations that we show can be overco...

A method for detection and identification of core antigen of hepatitis C virus (HCVcoreAg)-containing particles in the serum was proposed, with due account taken of the interactions of proteotypic peptides with Na(+), K(+), and Cl(-) ions. The method is based on a combination of reversible biospecific atomic force microscopy (AFM)-fishing and mass spectrometry (MS). AFM-fishing enables concentr...

Asymmetrical frequency modulation (AFM) as proposed by Palamin et al. (1988) offers greater flexibility in the shaping of the spectra of synthesized waveforms as compared to normal frequency-modulation (FM) synthesis. The feasibility of using AFM synthesis for the real-time generation of waveforms of musical instruments is demonstrated using a DSP56000 digital signal processor to implement the ...

The objectives of this study were to evaluate the usefulness of DArT markers in genotypic identification of willow species and describe genetic relationships between four willow species: Salix viminalis, S. purpurea, S. alba and S. triandra. The experimental plant material comprised 53 willow genotypes of these four species, which are popularly grown in Poland. DArT markers seem to identify Sal...

Scanning rates of the atomic force microscope (AFM) could be significantly increased by integrating the force sensing probe with microelectromechanical systems (MEMS). We present a micromachining method for batch fabrication of in-plane AFM probes that consist of an ultra-sharp silicon nitride tip on a single crystal silicon cantilever. Our fabrication method is fully compatible with the silico...

Atomic force microscopy (AFM) is a powerful and versatile tool for probing the mechanical properties of biological samples. This chapter describes the procedures for using AFM indentation to measure the elastic moduli of living cells. We include step-by-step instructions for cantilever calibration and data acquisition using a combined AFM/optical microscope system, as well as a detailed protoco...