BACKGROUND Saraphi (Mammea siamensis) is a Thai traditional herb. In this study, the cytotoxic effects of crude ethanolic and fractional extracts including hexane, ethyl acetate, and methanol fractions from M. siamensis flowers were investigated in order to determine their effect on WT1 expression in Molt4 and K562 cells and Bcr/Abl expression in K562 cells. MATERIALS AND METHODS The flowers ...

The K562 Chronic Myeloid Leukaemia Cell Line undergoes Apoptosis in Response to 4-chloro-5-iodo-7H-pyrrolo[2.3-d]pyrimidine

Human erythroid-potentiating activity (EPA) is a 28,000 mol wt glycoprotein that stimulates the growth of erythroid progenitors in vitro and enhances colony formation by the K562 human erythroleukemia cell line. EPA has potent protease inhibitory activity, and is also referred to as tissue inhibitor of metalloproteinases (TIMP). We observed that colony formation by K562 cells in semi-solid medi...

We previously reported that M\2, a new morpholino anthracycline, showed marked effects on pleiotropic drug-resistant sublines of murine P388 leukemia in vivo as well as in vitro. In this study we examine the in vitro cytotoxicity against pleiotropic drug-resistant sublines of human tumor cell lines. MX2 was effective against multidrug-resistant sublines of four human tumor cell lines; these cel...

Background: Identification of antigen-specific T cells has yielded valuable information on pathologic process and the disease state. Assays for quantification of inflammatory cytokines or lytic-granule molecules have been generally used to evaluate antigen specific T cell response, however their applicability have been hampered due to the limited source of autologous antigen-presenting target c...

The aim of the present study was to investigate the cytotoxic effect and multi-drug resistance (MDR) of Clostridium difficile toxin A (TcdA) on K562/A02 cells, and understand its underlying molecular pathways. K562/A02 cells were treated with TcdA at different concentrations for 24, 48 and 72 h, and the inhibition effect and drug resistance of TcdA on K562/A02 cell proliferation was assessed by...

Taking into account the special role of oxidative stress that increases during cancer chemotherapy, effect antioxidant emoxipine on peripheral blood mononuclears was studied under conditions simulate cytotoxic effects antimetabolites a number modified cytidine nucleosides in relation to tumor cell line K562. Lymphoid cells were also source for subsequent modelling immune response cancer. It fou...

Aim. Cancer continues to pose a serious threat human health. Euphorbia plants are rich in phenolics, aromatic esters, steroids and several bioactive compounds. Studies have shown the presence of large number compounds E. echinus including flavonoids, proanthocyanins. Method. There it was investigated cytotoxic effects methanolic extract on K562, HL60, Ishikawa, Raji SH-SY5Y cells. Results. The ...

The present study evaluated whether the magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) could enhance the activity of artesunate (ART), and to explore its potential mechanisms. Cytotoxicity of the copolymer of ART with MNPs-Fe(3)O(4) on K562 cells was detected by MTT assay and the apoptosis rate of K562 cells was measured by flow cytometry. Protein expression levels of bcl-2, bax, bcl-ramb...