I . Diets containing varying lcvels of crude protein (CP) in the range 9.5-18.5 "/b with and without supplements of diammonium citrate (DAC) as a source of non-protein nitrogen (KPN) were given to 160 growing chicks from z to 4 weeks of age, and their livers were assayed for glutamate dehydrogenase (GDH) activity. 2. Growth rate and total liver protein were increased by raising the protein leve...

Glucose, the main energy substrate used in the CNS, is continuously supplied by the periphery. Glutamate, the major excitatory neurotransmitter, is foreseen as a complementary energy contributor in the brain. In particular, astrocytes actively take up glutamate and may use it through oxidative glutamate dehydrogenase (GDH) activity. Here, we investigated the significance of glutamate as energy ...

NADP-dependent glutamate dehydrogenase (NADP-Gdh) is the first step in ammonia assimilation pathway in Saccharomyces cerevisiae and the knowledge of its regulation is the key for many biotechnological purposes such as single cell protein production. The regulation of NADP-Gdh activity in Kluyveromyces marxianus cells was evaluated under different ammonia supply in batch cultivations. The result...

Development of effective direct electron transfer is considered an interesting platform to obtain high performance bioelectrodes. Therefore, designing scalable and cost-effective immobilization routes that promotes correct electrical contacting between the electrode material redox enzyme still required. As we present here, electrochemical entrapment pyrroloquinoline quinone-dependent glucose de...

We propose a robust proactive threshold signature scheme, a multisignature scheme and a blind signature scheme which work in any Gap Diffie-Hellman (GDH) group (where the Computational Diffie-Hellman problem is hard but the Decisional Diffie-Hellman problem is easy). Our constructions are based on the recently proposed GDH signature scheme of Boneh et al. [BLS]. Due to the instrumental structur...

During chronic metabolic acidosis, the adaptive increase in rat renal ammoniagenesis is sustained, in part, by increased expression of mitochondrial glutaminase (GA) and glutamate dehydrogenase (GDH) enzymes. The increase in GA activity results from the pH-responsive stabilization of GA mRNA. The 3'-untranslated region (3'-UTR) of GA mRNA contains a direct repeat of an eight-base AU-rich elemen...

We studied gene expression in the ancient eukaryote, Giardia lamblia, by taking advantage of assays developed recently in our laboratory, which allow new genetic analyses of this organism. We examined the transcription of a 2.2-kilobase segment of the Giardia genome that contains the glutamate dehydrogenase (GDH) gene and a portion of a second open reading frame encoding an uncharacterized gene...

Glutamine synthetase (GS) and NADP-dependent glutamate dehydrogenase (NADP-GDH) play a key role in nitrogen assimilation in the ectomycorrhizal fungus Laccaria laccata (Scop. ex Fr. Cke) strain S 238. The two enzymes were purified to apparent electrophoretic homogeneity by a three-step procedure involving diethylaminoethyl (DEAE)-Trisacryl and affinity chromatography, and DEAE-5PW fast protein ...

We have developed a gene transfer system for the protozoan parasite Giardia lamblia. This organism is responsible for many cases of diarrhea worldwide and is considered to be one of the most primitive eukaryotes. Expression of a heterologous gene was detected in this parasite after electroporation with appropriate DNA constructs. We constructed a series of transfection plasmids using flanking s...

BACKGROUND Rapid commercial assays, including nucleic acid amplification tests and immunoassays for Clostridium. difficile toxins, have replaced the use of older assays. They are included in a two-step algorithm diagnosis, including first the detection of the glutamate dehydrogenase (GDH) as a screening method and second the detection of toxins as a confirmatory method. Although assays that det...