Access to point-of-care (POC), rapid, inexpensive, sensitive, and instrument-free tests for the diagnosis of tuberculosis (TB) remains a major challenge. Here, we report a simple and low-cost microchip-based TB ELISA (MTBE) platform for the detection of anti-mycobacterial IgG in plasma samples in less than 15 minutes. The MTBE employs a flow-less, magnet-actuated, bead-based ELISA for simultane...

Methyl tertiary butyl ether (MTBE) is an additive in some formulations of unleaded gasoline (UG) that enhances octane and reduces carbon monoxide emissions from motor vehicles. MTBE in CD-1 mice and UG in B6C3F1 mice increased the incidence of liver tumors selectively in female mice in their chronic bioassays. Both agents were negative in in vitro tests of genotoxicity, and exhibit similar hepa...

Methyl tert-butyl ether (MTBE) is a contaminant of concern to groundwater resources due to its persistence in subsurface environments. MTBE appears to be degraded readily in the presence of oxygen but is recalcitrant under the anaerobic conditions prevalent in the subsurface, and can be converted into the more toxic compound tert-butanol (TBA). As ethanol is being promoted as a renewable fuel a...

Methyl tertiary butyl ether (MTBE)-A well known gasoline additive substituting for lead alkyls-causes lipid disorders and liver dysfunctions in animal models. However, whether MTBE exposure is a risk factor for non-alcoholic fatty liver disease (NAFLD) remains uncertain. We evaluate the possible relationship between MTBE exposure and the prevalence of NAFLD among 71 petrol station attendants in...

A fuel component perturbation experiment was performed in order to determine the sensitivity of the engine-out emission levels of two olefins, 2-methylpropene (2MP) and 1,3-butadiene, to selected fuel components. In this experiment, a base fuel was perturbed with the following four major fuel components: MTBE, toluene, m,p-xylene, and iso-octane. For each of these components, new fuel mixtures ...

methyl tert-butyl ether (mtbe), a gasoline octane enhancer, was introduced as a substitute for lead tetraethyl over 30 years ago. widespread use of mtbe in gasoline, has introduced mtbe into the environment compartments, mostly into the under ground and surface water and water as a second most frequently detected contaminant. in this study, we have isolated pure cultures from bacterial consorti...

This research is focused on determination of toxicity, genotoxicity and discovery of MTBE-degrading bacterial strains. Toxicity was detected using bacterial luminescent method Microtox while genotoxicity was monitored by Ames test with Salmonella typhimurium his (TA 98, TA 100, YG 1041 and YG 1042) as indicator strains. Potential MTBE-degrading strains were tested by Bioscreen. This method moni...

Bifunctional aluminum is prepared by sulfating aluminum metal with sulfuric acid. The use of bifunctional aluminum to degrade methyl tert-butyl ether (MTBE) in the presence of dioxygen has been examined using batch systems. Primary degradation products were tert-butyl alcohol, tert-butyl formate, acetone, and methyl acetate. The initial rate of MTBE degradation exhibited pseudo-first-order beha...

A stand of five conifers (Pinus sp.) bordering a gasoline service station was studied to estimate the methyl tert-butyl ether (MTBE) emission rate from gasoline-impacted groundwater. Groundwater was impacted with gasoline oxygenates MTBE and tert-butyl alcohol (TBA) at combined concentrations exceeding 200,000 microg/L. Condensate from trees was collected in sealed environmental chambers and an...