ACE2 là một enzym thụ thể màng tế bào liên kết với protein gai (Spike-S) của virus SARS-CoV-2 và TMPRSS2 phân cắt S tại các vị trí S1 / S2 S'2, giúp dung hợp để xâm nhập vào bào. Do đó, mức độ biểu hiện này càng lớn có làm gia tăng sự nặng bệnh. Nghiên cứu khảo sát diễn biến đặc điểm lâm sàng, cận sàng 297 bệnh nhân COVID-19; tích so sánh tương đối mRNA bằng phương pháp realtime-PCR 52 mẫu dịch...

BACKGROUND & OBJECTIVES Rapid and accurate diagnosis and identification of Leishmania sp causing cutaneous leishmaniasis is crucial in control and therapeutic programs. The problem of diagnosis with traditional methods is that they have a low sensitivity or time consuming but molecular techniques would be an alternative method for rapid and accurate diagnosis. In this work, tryparedoxine peroxi...

ABSTRACT A multiplex real-time polymerase chain reaction (PCR) assay was developed to simultaneously genotype plants for the I and bc-1(2) alleles, which condition resistance in beans to Bean common mosaic virus and Bean common mosaic necrosis virus. A segregating F(2) population was derived from the cross between pinto bean breeding line P94207-189A (bc-1 bc-1 I I) and Olathe (bc-12 bc-1(2) i ...

In this article we report the identification of T-DNA (transfer DNA) insertion sites within two different gene regions in the genome of an Arabidopsis mutant line, SALK_084889. The T-DNA positions are in the 3' UTR (untranslated region) of DREB2A (Dehydration-responsive element-binding protein 2A) (AT5G05410) and promoter of LOX1 (Lipoxygenase 1) (AT1G55020) as determined by DNA-PCR and sanger ...

Panax ginseng is a well-known herbal medicine in traditional Asian medicine, and wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention. However, little has actually been reported on the difference between wild ginseng and cultivated ginseng. Thus, to identify and analyze those differences, we used suppressive subtraction hybridization (SSH) sequences with ...

To evaluate seasonal trivalent inactivated influenza vaccine effectiveness (VE) in Scotland, we performed a Scotland-wide linkage of patient-level primary care, hospital and virological swab data from 3,323 swabs (pooling data over nine influenza seasons: 2000/01 to 2008/09). We estimated the VE for reducing realtime RT-PCR-confirmed influenza using a test-negative study design. Vaccination was...

Background Tuberculosis is the second leading cause of death due to infectious disease worldwide. Delay in bacilli isolation in culture, cumbersome susceptibility testing methods, lack of methods for differentiation of Mycobacterium tuberculosis (MTB) from non tuberculous mycobacteria, are important issues which impinge on the WHO’s millennium goal of disease reduction by 2015. Traditional meth...