Stokes identified the phenomenon of fluorescence in the mid 19 century, and the first fluorescence microscopes were developed at the beginning of the 20th Century. These were used to study autofluorescence in organic and inorganic compounds. Imaging of secondary fluorescence (whereby specific tissues and bacteria which did not autofluoresce were labeled with a fluorescent marker and subsequentl...

The ferric reducing antioxidant power (FRAP) assay was recently adapted to a microplate format. However, microplate-based FRAP (mFRAP) assays are affected by sample volume and composition. This work describes a calibration process for mFRAP assays which yields data free of volume effects. From the results, the molar absorptivity (ε) for the mFRAP assay was 141,698 M(-1) cm(-1) for gallic acid, ...

Membrane viscosity is a key parameter in cell physiology, cell function, and cell signaling. The most common methods to measure changes in membrane viscosity are fluorescence recovery after photobleaching (FRAP) and fluorescence anisotropy. Recent interest in a group of viscosity sensitive fluorophores, termed molecular rotors, led to the development of the highly membrane-compatible (2-carboxy...

Plant foods are important due to their antioxidant activity (AOA) attributed to the phenolics which are known to protect organisms against harmful effects of oxygen radicals. However, information on antioxidant activity of Indian plant foods is scanty. Therefore, the present study evaluated the AOA of cereals, millets, pulses and legumes, commonly consumed in India and assessed the relationship...

Cells contain many subcellular structures in which specialized proteins locally cluster. Binding interactions within such clusters may be analyzed in live cells using models for fluorescence recovery after photobleaching (FRAP). Here we analyze a three-dimensional FRAP model that accounts for a single spatially localized cluster of binding sites in the presence of both diffusion and impermeable...

Dysregulated signal transduction from receptor tyrosine kinases to phosphatidylinositol 3-kinase (PI3K), AKT (protein kinase B), and its effector FKBP-rapamycin-associated protein (FRAP) occurs via autocrine stimulation or inactivation of the tumor suppressor PTEN in many cancers. Here we demonstrate that in human prostate cancer cells, basal-, growth factor-, and mitogen-induced expression of ...

Obtaining quantitative kinetic parameters from fluorescence recovery after photobleaching (FRAP) experiments generally requires a theoretical analysis of protein mobility and appropriate solutions for FRAP recovery derived for a given geometry. Here we provide a treatment of FRAP recovery for a molecule undergoing a combined process of reversible membrane association and lateral diffusion on th...

nowadays the researches have indicated the fact that using synthetic antioxidants as food additives in edible oils can have adverse effects on human health. so preparing antioxidation resources for reduction of oxidation stress effects is important. the aim of this project was, representing a natural antioxidant source with frap test. the present study optimized the ultrasound assisted extracti...

پژوهش حاضر به بررسی این مسأله می‌پردازد که چگونه نوعِ متد آموزشی می‌تواند بر میزان اضطراب زبانی دانشجویان زبان‌های خارجی تأثیر بگذارد. یک گروه 40 نفره از دختر و پسر در سطح پیش‌دانشگاهی به‌عنوان جامعه آماری انتخاب شدند. آموزش زبان فرانسه دو هفته‌ی اول، طریق گرامر-ترجمه انجام شد. پایان هفته تست تعیین مقیاس کلاس خارجی: FLCAS، بعمل آمد. دوم نوع رویکرد کنشی تغییر یافت. پس مجدداً فراگیران نتایج حاصله ن...

Clinopodium vulgare L. (Lamiaceae) was used in the traditional Bulgarian medicine for treatment of wounds, diabetes and gastric ulcers. The aim present study to evaluate antioxidant capacity extract (CVE) fractions from C. (CV) using DPPH, ABTS FRAP methods. Enzyme inhibitory activity against acetylcholinesterase, α -glucosidase -amylase also investigated. Rosmarinic acid as a positive control....