ing a H-atom from substrate. These and other kinetics studies demonstrated that PFL-AE could undergo multiple turnover events, with the 150 PFL activations per PFL-AE reported in Table 1 not the upper limit, but rather a number limited by the PFL:PFL-AE ratio in the steady-state kinetics assays. As can be seen from the data summarized in Table 1, PFL-AE is one of the few radical SAM enzymes dem...

SMSr/SAMD8 is an ER-resident ceramide phosphoethanolamine synthase with a critical role in controlling ER ceramides and suppressing ceramide-induced apoptosis in cultured cells. SMSr-mediated ceramide homeostasis relies on the enzyme's catalytic activity as well as on its N-terminal sterile α-motif or SAM domain. Here we report that SMSr-SAM is structurally and functionally related to the SAM d...

Understanding how electrons travel through organic matter is important in designing materials for organic microelectronics[1] and understanding biological electron-transport processes.[2, 3] Herein we describe a simple experimental procedure to measure rates of electron transport across organic thin films having a range of structures, compare the data for several types of films, and outline a t...

A method to solve stochastic partial differential equations on random domains consists in using a one to one random mapping function which transforms the random domain into a deterministic domain. With this method, the randomness is then supported by the constitutive relationship of the material. In this paper, this method is applied in electrokinetics in the case of scalar potential and vector...

In higher plants, most of the above-ground part ultimately derives from small populations of mitotic cells, called the shoot apical meristem (SAM). The SAM is initially formed during embryogenesis, when the basic body architecture of a plant is established (Jürgens, 1995). Once formed, the SAM plays central roles in postembryonic shoot organ formation. The SAM generates stems, leaves, and flora...

To measure the affective responses during high-intensity and moderate-intensity exercises, single-item questionnaires Feeling Scale (FS) Felt Arousal (FAS) are often used. The aim of present study was to examine validation German versions FS FAS by Maibach et al. (2020) for autogenic training (AT), which is an exercise addressing lowest end physical activation. Therefore, 224 participants (135 ...

Antipsychotic medications are associated with weight gain and adverse metabolic effects that complicate the treatment and management of schizophrenia. Olanzapine (OLZ) in particular is associated with significant weight gain and adverse metabolic effects. The present Phase 1, proof of concept, multicenter, randomized, double-blind, placebo-controlled study investigated the safety and effect on ...

OBJECTIVE To determine predictors of systolic anterior motion and left ventricular outflow tract obstruction (SAM/LVOTO) after mitral valve repair (MVRep) in patients with myxomatous mitral valve disease. BACKGROUND Mechanisms for the development of SAM/LVOTO after MVRep have been described; however, predictors of this complication have not been explored. We hypothesize that pre-MVRep transes...

Methionine restriction extends the lifespan of various model organisms. Limiting S-adenosyl-methionine (SAM) synthesis, the first metabolic reaction of dietary methionine, extends longevity in Caenorhabditis elegans but accelerates pathology in mammals. Here, we show that, as an alternative to inhibiting SAM synthesis, enhancement of SAM catabolism by glycine N-methyltransferase (Gnmt) extends ...

SAM (S-adenosylmethionine, also known as AdoMet) is well known as the methyl donor for the majority of methyltransferases that modify DNA, RNA, histones and other proteins, dictating replicational, transcriptional and translational fidelity, mismatch repair, chromatin modelling, epigenetic modifications and imprinting, which are all topics of great interest and importance in cancer research and...