We previously compared the structure and motility suppressive capacity of nm23-H1 by transfection of wild type and site-directed mutant forms into breast carcinoma cells. Wild type nm23-H1 and an nm23-H1(S44A) (serine 44 to alanine) mutant suppressed motility, whereas the nm23-H1(P96S), nm23-H1(S120G), and to a lesser extent, nm23-H1(S120A) mutant forms failed to do so. In the present study wil...

BACKGROUND AND OBJECTIVE The investigation concerning the B7-H1 expression in colorectal cancer cells is at an early stage. It is unclear whether B7-H1 expression may have diagnostic or prognostic value in colorectal carcinoma. Additionally, how B7-H1 is associated with the clinical features of colorectal carcinoma is not known. In order to investigate the relationship between B7-H1 and colorec...

Lateral mobility studies comparing native and mutated membrane proteins, combined with treatments that alter clathrin lattice structure, can measure membrane protein-coated pit interactions in intact cells (Fire, E., Zwart, D., Roth, M. G., and Henis, Y. I. (1991) J. Cell Biol. 115, 1585-1594). We applied this approach to study the interactions of the H1 and H2 human asialoglycoprotein receptor...

Condensed and late-replicating interphase chromatin in the Dipertan insect Chironomus contains a divergent type of histone H1 with an inserted KAP-KAP repeat that is conserved in single H1 variants of Caenorhabditis elegans and Volvox carteri. H1 peptides comprising the insertion interact specifically with DNA. The Chironomid Glyptotendipes exhibits a corresponding correlation between the prese...

Proof. Clearly, the image of F is HK by definition. To see that F is injective, suppose that F (h1, k1) = F (h2, k2). Then by definition h1k1 = h2k2. Thus h −1 2 h1 = k2k −1 1 . Since H is a subgroup, h−1 2 h1 ∈ H, and since K is a subgroup, k2k −1 1 ∈ K. Thus h−1 2 h1 = k2k −1 1 ∈ H ∩ K = {1}, and so h −1 2 h1 = k2k −1 1 = 1. It follows that h−1 2 h1 = 1, so that h1 = h2, and similarly k2k −1 ...

We study the intersection of finitely generated subgroups of free groups by utilizing the method of linear programming. We prove that if H1 is a finitely generated subgroup of a free group F , then the Walter Neumann coefficient σ(H1) of H1 is rational and can be computed in deterministic exponential time of size of H1. This coefficient σ(H1) is a minimal nonnegative real number such that, for ...

The ligation of programmed death-ligand 1 (B7-H1) to T cells results in the preferential production of interleukin 10 (IL-10). We investigated if B7-H1 would be up-regulated in HIV infection, a disease characterized by increased IL-10 production, by measuring B7-H1, B7-1 (CD80), and B7-2 (CD86) expression and mRNA in 36 HIV-infected patients and in 22 healthy controls (HCs). Results showed that...

H1 linker histones are highly abundant proteins that compact nucleosomes and chromatin to regulate DNA accessibility and transcription. However, the mechanisms that target H1 regulation to specific regions of eukaryotic genomes are unknown. Here we report fluorescence measurements of human H1 regulation of nucleosome dynamics and transcription factor (TF) binding within nucleosomes. H1 does not...