Escherichia coli isolates (n = 300) collected from six sites in subtropical Brisbane, Australia, prior to and after storm events were tested for the presence of 11 virulence genes (VGs) specific to diarrheagenic pathotypes. The presence of eaeA, stx(1), stx(2), and ehxA genes specific for the enterohemorrhagic E. coli (EHEC) pathotype was detected in 56%, 6%, 10%, and 13% of isolates, respectiv...

the µ (mu) opioid receptors, which mediate the effects of morphine, are widely distributed in brain. the purpose of this study was to design a simple expression system for rat µ-receptor in escherichia coli (bl21). in this laboratory study, rat µ-receptor cdna was isolated from pcdna3 vector using xba1 and hind3 restriction enzymes. pet-15b was digested by nco1 restriction enzyme. µ-receptor cd...

conclusions our results showed that broad-spectrum antibiotics such as imipenem, meropenem, fosfomycin, cefoperazone/sulbactam, and vancomycin would be the first line and the most effective drugs for the empirical treatment of urinary tract pathogens due to their higher resistance rates against other drugs like cephalexin, cephradine, ciprofloxacin, levofloxacin, and norfloxacin. patients and m...

conclusions the results of this study indicated that more than 90% of e. coli isolates harbored the fimh gene. the high binding ability of fimh could result in the increased pathogenicity of e. coli; thus, fimh could be used as a possible diagnostic marker and/or vaccine candidate. materials and methods a total of 140 isolated e. coli strains from patients with uti were identified using biochem...

conclusions it can be concluded that the rsm is an effective method for the optimization of recombinant protein expression using synthetic genes in e. coli. results the proposed optimum fermentation condition consisted of 7.81 g l-1 glucose, od 600 nm prior induction 1.66 and induction temperature of 30.27°c. the model prediction of 0.267 g l-1 of rhifn-β and 0.961 g l-1 of acetate at the optim...

conclusions the results of the present study indicate that brazilian propolis demonstrated in vitro anti-microbial efficacy against s. mutans and c. albicans. results the antimicrobial efficacy of hexidine against s. mutans and e. coli was higher compared to that of c. albicans. the brazilian propolis (p) mr showed antimicrobial action against s. mutans and c. albicans. the antimicrobial effica...

b ackground: in recent decades, extended spectrum beta-lactamase (esbl) producing bacteria have increased worldwide. the most important causative agents of nosocomial infections throughout the world, escherichia coli and klebsiella pneumoniae as main esbl-producing bacteria are so highly regarded. trends  in  the  treatment  of  infections  by such  bacteria  have  led  to  a  global concern. t...

background: introduction: enterotoxigenic escherichia coli (etec) is the most important bacterial cause of watery  travelers' diarrhea  in  developing countries. watery diarrhea is can cause serious life-threatening dehydration. etec was caused diarrhea by the secretion of two heat-labile enterotoxins (lts) and the heat-stable enterotoxins (sts) which increase intestinal secretion. routine labo...