We developed a near infrared (NIR) virtual intraoperative surgical photoacoustic microscopy (NIR-VISPAM) system that combines a conventional surgical microscope and an NIR light photoacoustic microscopy (PAM) system. NIR-VISPAM can simultaneously visualize PA B-scan images at a maximum display rate of 45 Hz and display enlarged microscopic images on a surgeon's view plane through the ocular len...

Generalized two-dimensional (2D) correlation analysis of visible/near-infrared (NIR) spectra was performed to characterize the spectral intensity variations of chicken muscles induced by either storage time/temperature regime or shear force values. The results showed that intensities of two visible bands at 445 and 560 nm increase with the storage temperature under identical treatment, possibly...

Heptamethine cyanines are favorable for fluorescence imaging applications in biological systems owing to their near-infrared (NIR) absorption and emission. However, it is very difficult to quench the fluorescence of NIR dyes by the classic photoinduced electron transfer mechanism due to their relatively high-lying occupied molecular orbital energy levels. Herein, we present a simple and effecti...

Fluorescence imaging in the second near-infrared window (NIR-II) allows visualization of deep anatomical features with an unprecedented degree of clarity. NIR-II fluorophores draw from a broad spectrum of materials spanning semiconducting nanomaterials to organic molecular dyes, yet unfortunately all water-soluble organic molecules with >1,000 nm emission suffer from low quantum yields that hav...

A variety of optical techniques utilizing near-infrared (NIR) light are being proposed for intraoperative breast tumor margin assessment. However, immediately following a lumpectomy excision, the margins are inked, which preserves the orientation of the specimen but prevents optical interrogation of the tissue margins. Here, a workflow is proposed that allows for both NIR optical assessment fol...

Near infrared photoimmunotherapy (NIR-PIT) is a newly developed cancer therapy that relies on the binding of a near-infrared antibody photoabsorber conjugate (APC) to a cancer cell. Subsequent exposure to NIR light selectively induces rapid necrotic cell death on target-expressing cells with minimal off-target effects. When treated with NIR-PIT, targeted cells become swollen, develop blebs and ...

OBJECTIVE We previously reported that near infrared (NIR) irradiation weakens frontalis muscle contraction resulting in brow ptosis. In addition, NIR irradiation non-thermally long-lasting thinning of the panniculus carnosus muscle in rats. We sought to determine whether NIR irradiation affects contraction of the corrugator supercilii muscle (CSM). METHODS Three to 5 rounds of NIR irradiation...

A new type of monoclonal antibody (mAb)-based, highly specific phototherapy (photoimmunotherapy; PIT) that uses a near infrared (NIR) phthalocyanine dye, IRDye700DX (IR700) conjugated with a mAb, has recently been described. NIR light exposure leads to immediate, target-selective necrotic cell death in vitro. Detecting immediate in vivo cell death is more difficult because it takes at least 3 d...

ISSN: 0967-0335 © IM Publications LLP 2012 doi: 10.1255/jnirs.973 All rights reserved In the early 1960s, Karl Norris and co-workers of the Agricultural Research Service of the USA Department of Agriculture introduced NIR spectroscopy (700–2500 nm) as an analytical technique for agricultural products. They recognised the potential of the diffuse reflectance measurement for rapid analysis of gra...

A recently created tissue demonstrative technique, in light of label-free close infrared (NIR) polarimetric reflectance imaging for the characterization of histopathological tests of lung cancer cells, is displayed. The design, calibration, and testing of an automated NIR polarimetric imaging framework, with accentuation on lung cancer location, are advanced in detail. The depicted electro-opti...