Five membrane proteins from Acholeplasma laidlawii have been previously purified on a large scale. These proteins have been used to establish the relationship between the precipitation lines obtained by crossed immunoelectrophoresis of solubilized cell membrane proteins from A. laidlawii in the presence of the neutral detergent Tween 20 or those obtained in the presence of the anionic detergent...

In a study of 188 patients and 109 controls, the detection of antibody by counterimmunoelectrophoresis was used as a diagnostic aid in human infections with Bacteroides fragilis. It was found that positive results indicated current infection and negative results were not conclusive. The method used was simple, rapid, and easily performed in a routine laboratory, but further work is needed to en...

Counter-current immunoelectrohporesis is a rapid sensitive method for detecting pneumococcal capsular antigens in sputum. A result can be obtained within 45 minutes. The optimum conditions for performing the test are given. Counter-current immunoelectrophoresis works with all the 33 pneumococcal antigens tested except type 14. Better precipitin lines are obtained when the gel-support is acid (p...

Ten serogroups of Legionella pneumophila have now been described.' Although the direct fluorescent antibody technique is widely used to identify and serologically differentiate L pneumophila isolates,2 some cross reactivity between serogroups3 and some staining of non-legionella organisms with legionella antisera4 have been reporrea. Several simple alternatives to the direct fluorescent antibod...

Subcellular fractions from the cotyledon obtained by differential and density gradient centrifugation, and extracts of total proteins from both cotyledon and axial tissues were analyzed by diethylaminoethyl cellulose chromatography, zone electrophoresis, ultracentrifugation, immunodiffusion, and immunoelectrophoresis. Fractionation and characterization of proteins in subcellular organelles of t...

The patients examined in this study were the same cohort as in an earlier study of morphologic features2’ but with additional follow-up. Between March 1978 and February 1982, 100 patients were studied just prior to initial chemotherapy for MM; all were followed for at beast 3.5 years. Sufficient information was available on all patients to allow clinical staging, and bone marrow plasma cell LI ...

The year 2006 marks the 50th anniversary of the discovery of cellulose acetate (CA) electrophoresis by Joachim Kohn, a pathologist at Queen Mary's Hospital in Roehampton, London. During a career in pathology that began in 1950 and spanned 37 years, Kohn published more than 50 papers in clinical laboratory medicine. He was the first to report the use of CA microbiology filters as solid supports ...

By use of crossed immunoelectrophoresis techniques, respiratory syncytial (RS) virus-specific precipitates were produced between RS virus cellular antigen [solubilized in tris(hydroxymethyl)aminomethane-glycine buffer, pH 9] and antiserum raised in rabbits against semipurified RS virus. When these precipitates were employed as antigens for further immunizations in rabbits, antibodies (anti-RSV-...

The techniques of agar immunoelectrophoresis and agar double diffusion were applied to the study of the antigenicity of beta- and alpha(1)-lipoproteins separated by ultracentrifugation from normal human sera. The effects of delipidation were also investigated. It was shown that beta- and alpha(1)-lipoproteins are antigenically distinct. For each class of lipoprotein studied, a single antigenic ...

Human placental coated vesicles have been purified by a method involving sucrose-density-gradient centrifugation and treatment with wheat-germ agglutinin. These preparations were free of contamination by placental microvillus fragments. Crossed immunoelectrophoresis demonstrated that the coated vesicles contained a single serum protein, which was identified as transferrin. This transferrin was ...