β-thalassemia results from point mutations or small deletions in the β-globin (HBB) gene that ultimately cause anemia. The generation of induced pluripotent stem cells (iPSCs) from the somatic cells of patients in combination with subsequent homologous recombination-based gene correction provides new approaches to cure this disease. CRISPR/Cas9 is a genome editing tool that is creating a buzz i...

Current methods that assay hemoglobin beta-globin chain variants can have limited clinical sensitivity when applied techniques identify only a predefined panel of mutations. Even sequence-based assays may be limited depending on which gene regions are investigated. We sought to develop a clinically practical yet inclusive molecular assay to identify beta-globin mutations in multicultural popula...

Two rapid, simple and sensitive extractive specrophotometric methods has been developed for the assay of Hyoscine butyle bromide (HBB), losartan potassium (LSR) and Sertaline HCl (SER) in bulk and in their pharmaceutical formulations. The proposed methods depend upon the reaction of cobalt(II)–thiocyanate (method A) and molybdenum(V)–thiocyanate ions (method B) with the cited drugs to form stab...

143) were transported 2,200 km to central Oklahoma. As an estimate of inflammation, concentrations of ceruloplasmin, fibrinogen, and haptoglobin were measured in blood samples collected at weaning, 24 and 72 h post-weaning, and on the day of shipping, 24 and 72 h after arrival. Fixed effects were breed type (n = 9 levels: straightbreds [RR, AA, BB], and crossbreds [RA, AR, RB, BR, AB, BA] where...

Intermediate beta-thalassemia has a broad spectrum of sequelae and affected subjects may require occasional blood transfusions over their lifetime to correct anemia. Iron overload in intermediate beta-thalassemia results from a paradoxical intestinal absorption, iron release from macrophages and hepatocytes, and sporadic transfusions. Pathological iron accumulation in parenchyma is caused by ch...

Sudden cardiac death (SCD), which is primarily caused by lethal heart disorders resulting in structural and arrhythmogenic abnormalities, is one of the prevalent modes of death in most developed countries. Myocardial ischemia, mainly due to coronary artery disease, is the most common type of heart disease leading to SCD. However, postmortem diagnosis of SCD is frequently complicated by obscure ...

Objective: Exercise and herbal medicine Berberine are known as anti-inflammatory agents. This study aimed to evaluate the effect of 4-weeks of endurance training and Berberine Chloride (BC) consumption on inflammatory factors and glycemic index in male wistar diabetic rats. Materials and Methods: In an experimental trial, 36 male wistar rats divided into 6 groups of 6 rats including 1) control...

<p><strong>Background: </strong>Tubal factor is responsible for most of the causes infertility, especially in our environment, and hysterosalpingography (HSG) investigation choice assessing tubal patency.<strong> </strong>Objective current study was to evaluate effect addition intramuscular hyoscine-N-butyl bromide (HBB) local systemic analgesics on pain perception...

In this paper, we show several known-plaintext attacks on the stream cipher HBB which was proposed recently at INDOCRYPT 2003. The cipher can operate either as a classical stream cipher in the “B mode” or as an asynchronous stream cipher in the “SS mode”. In the case of the SS mode, we present known-plaintext attacks recovering 128-bit key with the complexity 2 and 256-bit key with the complexi...