Dysregulated signal transduction from receptor tyrosine kinases to phosphatidylinositol 3-kinase (PI3K), AKT (protein kinase B), and its effector FKBP-rapamycin-associated protein (FRAP) occurs via autocrine stimulation or inactivation of the tumor suppressor PTEN in many cancers. Here we demonstrate that in human prostate cancer cells, basal-, growth factor-, and mitogen-induced expression of ...

Obtaining quantitative kinetic parameters from fluorescence recovery after photobleaching (FRAP) experiments generally requires a theoretical analysis of protein mobility and appropriate solutions for FRAP recovery derived for a given geometry. Here we provide a treatment of FRAP recovery for a molecule undergoing a combined process of reversible membrane association and lateral diffusion on th...

nowadays the researches have indicated the fact that using synthetic antioxidants as food additives in edible oils can have adverse effects on human health. so preparing antioxidation resources for reduction of oxidation stress effects is important. the aim of this project was, representing a natural antioxidant source with frap test. the present study optimized the ultrasound assisted extracti...

Clinopodium vulgare L. (Lamiaceae) was used in the traditional Bulgarian medicine for treatment of wounds, diabetes and gastric ulcers. The aim present study to evaluate antioxidant capacity extract (CVE) fractions from C. (CV) using DPPH, ABTS FRAP methods. Enzyme inhibitory activity against acetylcholinesterase, α -glucosidase -amylase also investigated. Rosmarinic acid as a positive control....

The antioxidant activity of aqueous extracts of fresh leaves of costmary (Chrysanthemum balsamita L.) was investigated. The effect of the extraction time on total phenolic compounds (TPhC) and antioxidant activity (ABTS -, CUPRACand FRAP assays) was studied. Results demonstrated highest levels of TPhC (0.60 GAE/g fresh plant weight), CUPRAC (5.93 μMTE/g FW), ABTS (10.68 μMTE/g FW), and FRAP (7....

background and objectives:alzheimer’s disease (ad) is a neurodegenerative disorder. nowadays, many investigations are performed to find new drugs for ad and medicinal plants are considered as one of the most important sources for developing new drugs. according to the role of oxidant agents and acetylcholinesterase enzyme (ache) in ad, plants with antioxidant and ache inhibition properties coul...

background and objectives:lallemantia iberica (lamiaceae) is a medicinal plant distributed in different parts of iran. this research, has evaluated the phytochemical constituents and antioxidant activity of the aerial parts of the plant. methods: different chromatographic methods such as column chromatographies using silica gel (normal and reversed phases), sephadex lh-20 and hplc were used for...