we consider the semilinear elliptic boundary value problem  = ∈∂ω− δ = ∈ωu x xu x f u x x( ) 0;( ) λ ( ( ));where λ > 0 is a parameter, ω is a bounded region in rn with a smooth boundary, and f is asmooth function. we prove, under some additional conditions, the existence of a positive solution for λlarge. we prove that our solution u for λ large is such that = →∞∈ω|| u ||: sup| u(x) |xas λ ...

Expression of V(D)J recombinase activity in developing lymphocytes is absolutely required for initiation of V(D)J recombination at antigen receptor loci. However, little is known about when during hematopoietic development the V(D)J recombinase is first active, nor is it known what elements activate the recombinase in multipotent hematopoietic progenitors. Using mice that express a fluorescent ...

Assessing the cell-type expression pattern of a certain gene can be achieved by using cell-type-specific gene manipulation. Recently, cre-recombinase-dependent gene-silencing tool, pSico has become popular in neuroscientific research. However, pSico has a critical limitation that gene-silenced cell cannot be identified by fluorescence, due to an excision of the reporter gene for green fluoresce...

We investigate the rare baryonic exclusive decays of Λb → Λll (l = e, μ, τ) with polarized Λ. Under the approximation of the heavy quark effective theory (HQET), in the standard model we derive the differential decay rates and various polarization asymmetries by including lepton mass effects. We find that with the long-distance effects the decay branching ratios are 5.3× 10 for Λb → Λll (l = e,...

BACKGROUND Group B streptococcal infections are the leading cause of sepsis and meningitis in newborns. A rapid and reliable method for the detection of this pathogen at the time of delivery is needed for the early treatment of neonates. Isothermal amplification techniques such as recombinase polymerase amplification have advantages relative to PCR in terms of the speed of reaction and simplici...

Site-specific recombinases are enzymes that promote precise rearrangements of DNA sequences. They do this by cutting and rejoining the DNA strands at specific positions within a pair of target sites recognized and bound by the recombinase. One group of these enzymes, the serine recombinases, initiates strand exchange by making double-strand breaks in the DNA of the two sites, in an intermediate...

We have determined the X-ray crystal structures of two DNA Holliday junctions (HJs) bound by Cre recombinase. The HJ is a four-way branched structure that occurs as an intermediate in genetic recombination pathways, including site-specific recombination by the lambda-integrase family. Cre recombinase is an integrase family member that recombines 34 bp loxP sites in the absence of accessory prot...

BACKGROUND The mycobacteriophage large serine recombinase Bxb1 catalyzes site-specific recombination between its corresponding attP and attB recognition sites. Previously, we and others have shown that Bxb1 has catalytic activity in various eukaryotic species including Nicotiana tabacum, Schizosaccharomyces pombe, insects and mammalian cells. RESULTS In this work, the Bxb1 recombinase gene wa...