An evaluation of several potential factors limiting carbon dioxide excretion by rainbow trout (Oncorhynchus mykiss) red blood cells was performed in vitro using a recently developed radioisotopic assay. Red blood cell (RBC) CO2 excretion was reduced by pretreatment (30min) of blood with the carbonic anhydrase inhibitor acetazolamide (final nominal concentration 1024 mol l21) or the Cl/HCO3 exch...

allura red (ar) is a widely used colorant in food industry, but there is debate on its potential security risk. in this study, in vitro inhibitory properties of the dye against carbonic anhydrase (ca) were evaluated. the esterase activity of purified ca decreased in the presence of ar, in a dose-dependent manner. regarding literature review and observed results, this preliminary study may provi...

PURPOSE To report the combination cocktail of strong steroid, non-steroidal anti-inflammatory drug (NSAID), and carbonic anhydrase inhibitor drops for treatment of cystoid macular edema. METHODS This is a retrospective case series of patients with cystoid macular edema managed with a topical combination of strong steroid (difluprednate), NSAID, and carbonic anhydrase inhibitor drops. The pati...

Human carbonic anhydrase C and bovine carbonic anhydrase B were modified with the affinity label, bromoacetazolamide, and human carbonic anhydrase B with N-bromoacetylacetazolamide. Tryptic peptides from the modified enzymes, containing alkylated histidines, have been isolated by ion exchange and two-dimensional high voltage electrophoresispaper chromatography. The partial amino acid sequence o...

The kinetics of catalysis of COZ hydration by human carbonic anhydrases B and C (EC 4.2.1.1) has been reinvestigated with use of an improved pH indicator stop-flow approach that was checked by studying the uncatalyzed rate of hydration. The Michaelis-Menten parameters were determined between pH 5.8 and 8.8 in noninhibitory buffers. For both isoenzymes K, was independent of pH, whereas V max inc...

binary logistic regression (blr) has been developed as non-linear models to establish quantitative structure- activity relationships (qsar) between structural descriptors and biochemical activity of carbonic anhydrase inhibitors. using a training set consisted of 21 compounds with known ki values, the model was trained and tested to solve two-class problems as active or inactive on the basis of...

It was previously reported that magnesium ion inhibited carbonic anhydrase (Bamberger and Avron 1975 Plant Physiol 56: 481-485). Studies with partially purified carbonic anhydrase from spinach (Spinacia oleracea L.) chloroplasts show that the effect was the result of the chloride counterion and not the magnesium ion. Enzyme activity was reduced 50% upon addition of 3 to 10 millimolar MgCl(2) or...

This study focused on the physiological significance of carbonic anhydrase 8 (CAR8) in GLP-1 secretion from enteroendocrine preproglucagon (PPG)-expressing cells. We found an inhibitory role CAR8 LCFA-induced vitro and vivo, suggesting a novel therapeutic approach to diabetes obesity through augmentation postprandial by inhibition.

The widely accepted models for the role of carboxysomes in the carbon-concentrating mechanism of autotrophic bacteria predict the carboxysomal carbonic anhydrase to be a crucial component. The enzyme is thought to dehydrate abundant cytosolic bicarbonate and provide ribulose 1.5-bisphosphate carboxylase/oxygenase (RubisCO) sequestered within the carboxysome with sufficiently high concentrations...