Zymomonas mobilis is a Gram-negative bacterium that has drawn attention in the bioethanol industry. Besides bioethanol, this bacterium also produces other biotechnological products such as levans, which show antitumor activity. Molecular studies involving Z. mobilis have advanced to the point that allows us to characterize interspecies genetic diversity and understand their metabolism, and thes...

A mathematical model is described for the simultaneous saccharification and ethanol fermentation (SSF) of sago starch using amyloglucosidase (AMG) and Zymomonas mobilis. By introducing the degree of polymerization (DP) of oligosaccharides produced from sago starch treated with c~-amylase, a series of MichaelisMenten equations were obtained. After determining kinetic parameters from the results ...

We have developed a number of broad-host-range plasmids that allow the expression of the Escherichia coli lac operon from any cloned promoter, and the creation of 'in phase' fusions between lacZ and other cloned genes. In a second series of constructions, the E. coli gal operon has been cloned into the broad-host-range vector and a plasmid carrying both the E. coli gal and lac genes is describe...

Streptomyces strains are producing many important secondary metabolites, in many cases used as pharmaceutical drugs. Though the usage of these bacteria for the production of fuel has never been described. We could show, that the expression of two artificial ethanologenic genes of Zymomonas mobilis in different actinomycetes strains resulted in the production of ethanol. The synthetic genes adhB...

Biotechnological processes represent a challenge into the control field, due to its high non linearity. Particularly, continuous alcoholic fermentation by Zymomonas mobilis presents high ethanol performance, but it shows highly oscillatory behaviour in process variables, because of the influence of inhibition dynamics (rate of ethanol concentration Z and I) over Biomass, Substrate and Product c...

The expression of the ice nucleation gene inaZ from Pseudomonas syringae in Zymomonas mobilis strains under the control of three different promoters was investigated to establish the utility of the gene as a reporter and examine the possible use of the organism as a source of ice nuclei for biotechnological applications. A promoterless version of the inaZ gene was placed under the control of th...

The sacB and sacC genes encoding levansucrase and extracellular sucrase respectively were independently subcloned in pBluescript (high copy number) and in Z. mobilis-E. coli shuttle vector, pZA22 (low copy number). The expression of these genes were compared under identical background of E. coli and Z. mobilis host. The level of sacB gene expression in E. coli was almost ten fold less than the ...