Bioethanol is an alternative energy source that increasingly needed along with the depletion of petroleum stocks. can be produced by utilizing various wastes, including jackfruit peel and a mixture crude cellulose enzymes from Trichoderma reesei Aspergillus niger as well fermentation using Saccharomyces cerevisiae Zymomonas mobilis. This study aimed to compare production bioethanol S. Z. mobili...

This work presents the development of an unstructured kinetic model incorporating the differing degrees of product inhibition on the kinetic rates of recombinant Zymomonas mobilis fermentation. A mechanism for the inhibition of two competing substrates cellular uptake via membrane transport is proposed. The model was utilized in a nonlinear model predictive control (NMPC) algorithm to control p...

The genes encoding essential enzymes of the fermentative pathway for ethanol production in Zymomonas mobilis, an obligately ethanologenic bacterium, were inserted into Escherichia coli under the control of a common promoter. Alcohol dehydrogenase II and pyruvate decarboxylase from Z. mobilis were expressed at high levels in E. coli, resulting in increased cell growth and the production of ethan...

Zymomonas mobilis subsp. mobilis is one of the most rigorous ethanol-producing organisms known to date, considered by many to be the prokaryotic alternative to yeast. The two most applied Z. mobilis subsp. mobilis strains, ZM4 and CP4, derive from Recife, Brazil, and have been isolated from sugarcane fermentations. Of these, ZM4 was the first Z. mobilis representative strain to be sequenced and...

Mutant strain of the facultatively anaerobic, ethanol-producing bacterium Zymomonas mobilis, deficient in the Fe-containing alcohol dehydrogenase isoenzyme (ADH II), showed impaired homeostasis of the intracellular NAD(P)H during transition from anaerobic to aerobic conditions, and also in steady-state continuous cultures at various oxygen supplies. At the same time, ADH II deficiency in aerobi...

Pyruvate decarboxylase (EC 4.1.1.1) from Zymomonas mobilis purified to homogeneity by using dye-ligand and ion-exchange chromatography. Antibodies produced against the enzyme and the amino-terminal sequence obtained for the pure enzyme were used to select and confirm the identity of a genomic clone encoding the enzyme selected from a genomic library of Z. mobilis DNA cloned into pUC9. The genom...

The first synthesis of the cyclopentitol units in bacterial hopanoids has been accomplished from D-glucosamine and the possible biological activity of the free cyclitols as glycosidase inhibitors has been studied.

BACKGROUND The Rapid Bioconversion with Integrated recycle Technology (RaBIT) process reduces capital costs, processing times, and biocatalyst cost for biochemical conversion of cellulosic biomass to biofuels by reducing total bioprocessing time (enzymatic hydrolysis plus fermentation) to 48 h, increasing biofuel productivity (g/L/h) twofold, and recycling biocatalysts (enzymes and microbes) to...