We describe the design and synthesis of a new Tc-99m labeled bioconjugate for imaging activated complement, based on Short Consensus Repeats 1 and 2 of Complement Receptor 2 (CR2), the binding domain for C3d. To avoid non specific modification of CR2 and the potential for modifying lysine residues critical to the CR2/C3d contact surface, we engineered a new protein, recombinant CR2 (rCR2), to i...

Transcription of the human complement receptor type 2 (CR2/CD21) gene is controlled by both proximal promoter and intronic elements. CR2 is primarily expressed on B cells from the immature through mature cell stages. We have previously described the presence of an intronic element that is required for both cell- and stage-specific expression of CR2. In this study, we report the identification o...

Surface receptors specific for either the C4b (CR1) or C3d (CR2) component of complement were examined on the neoplastic cells from 30 cases of non-Hodgkin's lymphoma of B-cell origin and on cells derived from 9 normal lymphoid tissues. Lymphocyte suspensions from non-neoplastic peripheral blood, tonsils, and lymph node contained three categories of complement receptor lymphocytes (CRL): cells ...

Previous studies indicate a pivotal role for complement in mediating both local and remote injury following ischemia and reperfusion of the intestine. Here, we report on the use of a mouse model of intestinal ischemia/reperfusion injury to investigate the strategy of targeting complement inhibition to sites of complement activation by linking an iC3b/C3dg-binding fragment of mouse complement re...

Rare earth (RE) Tb(3+)-doped high quality ZnSe nanocrystals (NCs) were synthesized by a facile chemical hot-injection method. ZnSe:Tb(3+) nanocrystals exhibited broadband absorption below the first excitonic absorption peak. Photoluminescence spectra showed Tb(3+) emission lines in the visible spectral window at room temperature when excited by UV radiation below the band-edge of the host ZnSe ...

CdSe/ZnSe quantum structures grown on GaAs~001! by molecular-beam epitaxy were systematically investigated by high-resolution x-ray diffraction and high-resolution transmission-electron microscopy. Half of the initial Cd deposit redesorbs when migration-enhanced epitaxy is used instead of conventional molecular-beam epitaxy for the overgrowth of the CdSe by ZnSe. This result is explained by a s...

The purpose of this research is to evaluate new materials as gain media for midinfrared (MIR) solid-state lasers. MIR lasers are of significant current interest for several applications including laser remote sensing of bio-chemical agents, medical surgery, fundamental spectroscopy, and biophotonics. In this project, Cobalt (Co) doped ZnSe windows and CdTe crystals were investigated as MIR lase...

We measured the polarized and azimuthal dependencies of optical second harmonics generation (SHG) on polished surfaces of ZnSe(100) single crystal surface in air, using a fundamental wavelength of 1.06μm. By considering both, the bulkand surfaceoptical nonlinearities within the electric dipole approximation, we analysed the data for all four combination of pand s-polarized incidence and output....

Controlled growth of ZnSe and ZnCdSe nanowires is demonstrated by molecular beam epitaxy using Au or Ag catalyst films in the temperature range 400–550 ◦C. The highest density of small-diameter (10 nm), highly-crystalline ZnSe nanowires is achieved by using Au at 400 ◦C. Direct growth onto transmission electron microscope grids clearly indicates a tip-growth regime. Pre-patterning of the cataly...

pendence of time-resolved behaviors of another emission in ZnSe thin film at 470 nm. As the excitation power is increased, the faster component of duration less than 1 ps is also increased. This clearly indicates that the fast components are attributed to the stimulated emission from ZnSe under optical-pumping at 400 nm. The time decay and the delay relative to the pumping pulse of the emission...