The balance between pro- and anti-inflammatory cytokine production is a crucial aspect of infections caused by Candida albicans. We therefore investigated the effect of yeast concentration on the pro- and anti-inflammatory cytokine response. Production of tumor necrosis factor-alpha (TNF-α) by the murine macrophage cell line J774 decreased significantly as the live yeast concentration in...

Increasing interest in new beer types has stimulated the search for approaches to extend the metabolic variation of brewers' yeast. Therefore, we tested two approaches using non-conventional yeast to create a beer with lower ethanol content and a complex aroma bouquet. First, the mono-culture performance was monitored of 49 wild yeast isolates of Saccharomyces cerevisiae (16 strains), Cyberlind...

We present a fully automated setup for performing in-line mass spectrometry (MS) analysis of conditioned media in cell cultures, in particular focusing on the peptides therein. The goal is to assess peptides secreted by cells in different culture conditions. The developed system is compatible with MS as analytical technique, as this is one of the most powerful analysis methods for peptide detec...

Sodium bicarbonate and yeast culture effects on ruminal fermentation, intake, and growth were evaluated in young calves. In trail 1, nine ruminally cannulated Holstein calves averaging 12 wk of age were fed control starter (17% CP) or starters containing 3% sodium bicarbonate or .2% yeast (Saccharomyces cerevisiae) culture in a 3 x 3 Latin square. Calves were fed for ad libitum consumption for ...

This study is focussed on the possibility of producing a yeast culture with yellow wine lees as a substrate by solid-state fermentation (SSF). Results showed that a yeast count of 1.58 × 109 CFU/g was achieved by signal factor and orthogonal experiments. After fermentation, the starch content in the yeast culture reduced from 32.2% ± 0.5% to 7.5% ± 0.2%, and the contents of crude protein and pe...

The taxonomic nomenclature of Stelling-Dekker (1931) and Lodder (1934) is used throughout the paper. The yeast strains were derived partly from the stock of the Department of Bacteriology and Hygiene of the Hebrew University and partly from the following type culture collections: National Type Culture Collection, London; American Type Culture Collection, Washington; and the Centraalbureau voor ...

Corrosion of structural steel in an SRB containing environment for as long as 9 months, is studied using the Electrochemical Noise Analysis technique. The results show that the activity of the system, which was very low initially, started to increase after 6 months exposure to the environment. The power spectral density curves of the Electrochemical Potential Noise of the system, using the maxi...

BacT/Alert FAN blood culture bottles have been shown to enhance the recovery of bacteria and yeast from blood compared with standard BacT/Alert bottles. It is well established that standard BacT/Alert blood culture bottles require no more than 5 days of incubation for the detection of routine bacteria and yeast. It is less clear, however, whether FAN bottles also routinely require 5 days of inc...

Production of extracellular amylase was demonstrated under conditions of high salinity in aerobically cultivated culture of a newly isolated moderately halophilic Gram-positive coccus, designated strain F in basal medium containing peptone from meat, yeast extract, NaCl (7% w/v) and starch. Biochemical and physiological characterization along with 16S rRNA sequence analysis placed F in the genu...