Abstract As a typical triazole fungicide, penconazole (PEN) is widely used in agriculture but has been proven to be toxic. In this study, we designed new hapten prepare highly sensitive and specific anti-PEN monoclonal antibody (mAb) established gold nanoparticle-based lateral-flow immunoassay (LFIA) for the detection of PEN residues watermelon cucumber. The 50% inhibitory concentration (IC50) ...