UNLABELLED We provide evidence to show that the standard reactant concentrations used in tissue engineering to cross-link collagen-based scaffolds are up to 100 times higher than required for mechanical integrity in service, and stability against degradation in an aqueous environment. We demonstrate this with a detailed and systematic study by comparing scaffolds made from (a) collagen from two...

In this study, we report the preparation, luminescence, and targeting properties of folic acid-CdTe quantum dot conjugates. Water-soluble CdTe quantum dots were synthesized and conjugated with folic acid using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-N-hydroxysuccinimide chemistry. The influence of folic acid on the luminescence properties of CdTe quantum dots was investigated, and no ene...

Epidermal Growth Factor (EGF, Mw=6,045) has been reported to have high efficiency of wound repair and anti-wrinkle effect. However, the half-life of EGF in the body is too short to exert the biological activity effectively when applied in free form. Growth Factors can be stabilized by immobilization with carbohydrates from thermal and proteolytic degradation. Low molecular weight chitosan (LMCS...

A low molecular weight RNA was isolated from human cells infected with adenovirus 2 and its nucleotide sequence deduced to be ,G-G-G-C-A-C-U-C-U-U-C-C-G-U-G-G-UC-U-G-G-U-G-G-A-U-A-A-A-U-U-C-G-C-A-A-G-G-G-U-AU-C-A-U-G-G-C-(G-U-G-G-A-C-G-A-C-C)-G-G-G-G-U-UC-G-A-A-C-C-C-C-G-G-A-U-C-C-G-U-G-A-U-C-C-A-U-GC-G-G-U-U-A-C-C-G-U-C-C-G-C-C-G-C-C-C-G-U-G-C-GU-C-G-A-A-C-C-C-A-G-G-U-G-U-G-C-G-A-C-G-U-C-AG-A-...

Various functional groups of I~-lactamase I were modified using appropriate group specific reagents. Modification of five of the ten arginine residues and approximately fifteen of twenty-one lysine residues present in the protein could readily be achieved by reaction with diacetyl trimer and trinitrobenzene sulfonic acid respectively, without any adverse effect on catalytic activity. Reaction w...