نتایج جستجو برای: was amplified and sequenced using conventional rt
تعداد نتایج: 17375690 فیلتر نتایج به سال:
abstract part one: the electrode oxidation potentials of a series of eighteen n-hydroxy compounds in aqueous solution were calculated based on a proper thermodynamic cycle. the dft method at the level of b3lyp-6-31g(d,p) was used to calculate the gas-phase free energy differences ,and the polarizable continuum model (pcm) was applied to describe the solvent and its interaction with n-hydroxy ...
abstract while task-based instruction is considered as the most effective way to learn a language in the related literature, it is oversimplified on various grounds. different variables may affect how students are engaged with not only the language but also with the task itself. the present study was conducted to investigate language and task related engagement on the basis of the task typolog...
background: follicle stimulating hormone (fsh) plays an essential role in reproductive physiology and follicular development. objective: a new variant of the equine fsh (efsh) gene was cloned, sequenced, and expressed in pichia pastoris (p. pastoris) gs115 yeast expression system. materials and methods: the full-length cdnas of the efshα and efshβ chains were amplified by reverse transcription ...
objective: in this project, our aim was to construct a novel expressing vector harboring a new sequence from overlapping region of ns3 gene of hcv from infected iranian patient. materials and methods: the partial ns3 (pns3) gene was amplified by nested-rt-pcr method using sera of hcv infected patients harboring genotype 1a. after purification and cloning the pns3 into ta-cloning vector, the be...
objective: the objective of this study was to investigate the invitro fluoride release of four new self-adhesive resin cements; set (sdi, australia), breeze (pentron, usa), embrace wetbond (pulpdent, usa), g-cem (gc, japan) and to assess the bonding performance of these self-adhesive resin cements for bonding of orthodontic brackets. materials and methods: for fluoride release experiment, six ...
the rationale behind the present study is that particular learning strategies produce more effective results when applied together. the present study tried to investigate the efficiency of the semantic-context strategy alone with a technique called, keyword method. to clarify the point, the current study seeked to find answer to the following question: are the keyword and semantic-context metho...
background and aims: certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. reverse transcription-polymerase chain reaction (rt-pcr) has been documented as an alternative assay for certificat...
during 2010-2011, real-time pcr procedure was used to detecting fmdv rna on 147 epithelium samples from the field. in this survey, for real-time pcr from 3d gene segment as conserve region selected for tracking all of seven serotypes fmdv. the assay detected the viral rna in all serotypes of fmdv. the rrt-pcr specifically detected fmd virus in sample with greater sensitivity than our convention...
beet yellows virus (byv) belongs to the genus closterovirus, closteroviridae family, is one the most important viruses of sugar beet worldwide. in order to identify and characterize some molecular aspects of the byv, a total of 75 symptomatic leaf samples of sugar beet were collected from sugar beet field of west azarbayjan, hamedan and kermanshah provinces, iran. leaves showing general yellowi...
abstract in order to detect onion yellow dwarf virus in khorasan province in iran, onion samples from major onion growing regions where collected in spring and summer 2010. to determine the distribution of the virus, samples were randomly taken from onion fields in vicinity of mashhad, chenaran, quchan, torbate heydarye, neyshabur, bardaskan, gonabad, fariman, faruj, shirvan, bojnourd and ash k...
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