A rapid (<20min) gel-membrane biochip platform for the detection and quantification of short nucleic acids is presented based on a sandwich assay with probe-functionalized gold nanoparticles and their separation into concentrated bands by depletion-generated gel isotachophoresis. The platform sequentially exploits the enrichment and depletion phenomena of an ion-selective cation-exchange membra...

BACKGROUND Effective lung volume (ELV) for gas exchange is a new measure that could be used as a real-time guide during controlled mechanical ventilation. The authors established the relationships of ELV to static end-expiratory lung volume (EELV) with varying levels of positive end-expiratory pressure (PEEP) in healthy and surfactant-depleted rabbit lungs. METHODS Nine rabbits were anestheti...

The importance of plasma and extracellular fluid volumes in the mechanism of the antihypertensive effect of chlorothiazide is disputed. The present investigation indicates that the lowering of blood pressure is accompanied by reductions in plasma and extracellular fluid volumes and in body weight. Furthermore, re-expansion of plasma volume with salt-free dextran reverses the antihypertensive ef...

The turbulent coagulation of micron-sized colloidal particles in a liquid is controlled by the shearing motion produced by Kolmogorov scale eddies. The rate of energy dissipation has fluctuations which vary over the integral length and time scale in a homogeneous, isotropic turbulent flow. We present a model of the particle concentrations and coagulation rates in a set of fluid packets that are...

On the base of the 17 GHz radio maps of the Sun taken with the Nobeyama Radio Heliograph we estimate plasma parameters in the specific region of the sunspot atmosphere in the active region AR 11312. This region of the sunspot atmosphere is characterized by the depletion in coronal emission (soft X-ray and EUV lines) and the reduced absorption in the a chromospheric line (He I 1.083 μm). In the ...

ayurveda, the indian art of holistic medicine describes fasting in terms of upavasa and considers it as one among the depletion therapies.   besides the extensive explanations on the preventive purports of fasting, the classics have explicitly explained and exemplified the therapeutic importance of fasting while treating different diseases and dealing with different stages of the diseases. feve...

BACKGROUND There is no consensus regarding the blood volume that could be safely donated by dogs, ranging from 11 to 25% of its total blood volume (TBV). No previous studies evaluated sedated donors. AIM To evaluate the hemodynamic effects of blood collection from sedated and non-sedated dogs and to understand if such effects were volume-dependent. MATERIALS AND METHODS Fifty three donation...

Long-term head-down-tilt bed rest (HDT) causes cardiovascular deconditioning, attributed to reflex dysfunctions, plasma volume reduction, or cardiac impairments. Our objective with the present study was to evaluate the functional importance and relative contribution of these during rest and exercise in supine and upright postures. We studied six subjects before (baseline), during [days 60 (D60)...

The term cerebral salt wasting (CSW) was introduced before the syndrome of inappropriate antidiuretic hormone secretion was described in 1957. Subsequently, CSW virtually vanished, only to reappear a quarter century later in the neurosurgical literature. A valid diagnosis of CSW requires evidence of inappropriate urinary salt losses and reduced "effective arterial blood volume." With no gold st...

The interaction between two spheres in a solution of nonadsorbing polymers, with excluded volume interaction, is calculated from the depletion layers around the spheres using the generalized Gibbs adsorption equation. By combining the bulk correlation length with the curvature-dependent interfacial tension between a sphere and the surrounding polymer solution [Hanke, Eisenriegler, and Dietrich,...