We analyzed the emergence of daptomycin nonsusceptibility in a patient with persistent vancomycin-intermediate Staphylococcus aureus (VISA) bacteremia. The daptomycin-nonsusceptible VISA's cell wall demonstrated a reduction in muramic acid O-acetylation, a phenotypic parameter not previously reported for VISA; some isolates also contained a single point mutation in the mprF gene.

We introduce an end-to-end framework for data quality that integrates business strategy, data quality models, and supporting investigative and governance processes. We also describe a supporting IT architecture for this framework. Finally, we describe how this framework was implemented at Visa International Service Association (“Visa”) and some of the lessons learned during its use over the pas...

We describe here the genetic analysis of a vancomycin-susceptible Staphylococcus aureus (VSSA) strain, Mu50Omega, a strain related to vancomycin-intermediate S. aureus (VISA) strain Mu50. Using a combination of Mu50Omega whole-genome sequencing and genome engineering, we observed a stepwise evolution of vancomycin resistance from VSSA to VISA after the mutated vraS and graR genes of Mu50 were e...

Twenty-three isolates of Staphylococcus aureus resistant to methicillin have been analyzed, being found a clinical isolate of VISA through microdilution technique. The others techniques were unable to detect such isolates. This is the first study that shows the presence of VISA in clinical isolates in the city of Santa Maria-RS.

To the Editor:—As a junior faculty member committed to becoming an anesthesiology physician scientist, I read the article by Schwinn and Balser on recruiting and training more of my own with great interest. However, one issue not touched upon either in the article or in the accompanying Editorial View is that a large portion of recent anesthesiology graduates, including myself, do not hold US c...

Worst-case execution times (WCET) of tasks are essential for safe scheduling in hard real-time systems. However, contemporary processors exceed the capabilities of static worst-case timing analysis tools. The Virtual Simple Architecture (VISA) framework shifts the burden of bounding the WCET of tasks, in part, to hardware. A VISA is the pipeline timing specification of a hypothetical simple pro...

Emerging infections caused by vancomycin-intermediate Staphylococcus aureus (VISA) isolates are more likely to be associated with treatment failures than infections caused by other types of S. aureus. We present a case of pacemaker lead infective endocarditis caused by a non-daptomycin-susceptible strain of VISA. After 8 weeks of parenteral telavancin therapy, the patient achieved microbiologic...

To the Editor:—As a junior faculty member committed to becoming an anesthesiology physician scientist, I read the article by Schwinn and Balser on recruiting and training more of my own with great interest. However, one issue not touched upon either in the article or in the accompanying Editorial View is that a large portion of recent anesthesiology graduates, including myself, do not hold US c...

BACKGROUND There is no disease specific, reliable, and valid clinical measure of Achilles tendinopathy. OBJECTIVE To develop and test a questionnaire based instrument that would serve as an index of severity of Achilles tendinopathy. METHODS Item generation, item reduction, item scaling, and pretesting were used to develop a questionnaire to assess the severity of Achilles tendinopathy. The...