Hereditary deficiency of the macromolecular Factor VIII complex results in classic von Willebrand disease in man and animals, a bleeder state characterized by loss of the multiple biologic activities associated with the Factor VIII complex, including the platelet-aggregating von Willebrand factor. The bleeding time is also long. Venom coagglutinin, a Bothrops factor that causes platelet aggrega...

Hemophilia A is an X-linked bleeding disorder caused by a deficiency or abnormality of factor VIII, affecting approximately 1 male in 10,000. A subgroup of the patients develops inhibitors against factor VIII during substitution therapy. Because a considerable percentage of all cases is thought to result from de novo mutations, it is likely that many different molecular lesions lead to hemophil...

Rabbits were injected with an immunoglobulin fraction of human serum containing a factor VIII antibody. Factor VIII levels fell abruptly, persisted below 10% of a rabbit plasma standard for 12 hr. and returned to normal by 120-168 hr. The factor VIII antigen-antibody reaction did not result in Intravascular clotting as evaluated by kinetic studies with 125l-fibrinogen. However. small falls in f...

We prepared a highly purified and relatively heat stable form of factor VIII which contained 25 units per mL (u/mL) activity using PEG-4000 and developed an effective and new manufacturing process. Heat treatment was performed at 80°C for 72 hrs in the presence of different stabilizers. In our studies, we used different organic solvents as preservatives to maintain factor VIII activity, sin...

disorder due to deficiency of factor VIII and IX respectively. However, 1/3rd cases are due to spontaneous mutations. These patients present with bleeding. The platelet count, BT and PT are normal but APPT is deranged. Correction of APTT on mixing with normal plasma suggests intrinsic pathway defect. Normal factor levels are between 50% -150%. Hemophilia is defined as mild (factor levels >5% to...

Central nervous system hemorrhage is an uncommon and potentially devastating event in hemophiliacs, with an incidence of 2-8%.1 Intraspinal hematomas account for 8.5% of CNS hemorrhages in patients with factor VIII or IX deficiencies.2 Although most reported cases in this population are secondary to trauma, there are a few reports of spontaneous hemorrhage.3,4,5 We present a case of spontaneous...