Vibrio vulnificus was found to produce a chemical that induced the expression of Vibrio fischeri lux genes. Electron spray ionization-mass spectrometry and 1H nuclear magnetic resonance analyses indicated that the compound was cyclo(L-Phe-L-Pro) (cFP). The compound was produced at a maximal level when cell cultures reached the onset of stationary phase. Sodium dodecyl sulfate-polyacrylamide gel...

One of the major threats to seabass (Dicentrarchus labrax) aquaculture sector is development vibriosis, a disease caused mainly by bacteria from family Vibrionaceae, especially Vibrio harveyi. Given relationship between abundance in surrounding environment and prevalence fish, monitoring presence V. harveyi rearing water biofilms over time may help better understand dynamics pathogen factors th...

Early and precise pathogen identification corresponding disease management are primary concerns in aquaculture. Here, we attempted at diagnostic methods that can simultaneously identify multiple pathogens, where many samples, several concurrent infections to be handled. Hence, a multiplex PCR assay targeting five major aquaculture viz. Vibrio parahaemolyticus, anguillarum, alginolyticus, vulnif...

Vibrio harveyi infection was an obstacle in shrimp culture. Meanwhile, the use of antibiotics disease control no longer effective, so immunostimulants such as meniran leaves safest alternative. The purpose this study to determine effect adding different doses leaf extract (Phyllanthus niruri L.) feed on immune system vannamei (Litopenaeus vannamei) which challenged with harveyi. This research e...

This study was conducted to investigate the use of fresh and heat-treated roselle meals on growth, body colouration, survival against Vibrio harveyi infection whiteleg shrimp, Litopenaeus vannamei. Fresh (FR) or (HR) were added at 10% in isoproteic (38%) isolipidic (7%) diets. A control diet prepared without any addition meal. These diets fed shrimp (initial weight 1.26g±0.02g) triplicate group...

In Vibrio fischeri, the autoinducer N-3-oxohexanoyl-L-homoserine lactone (AI-1) governs the cell density-dependent induction of the luminescence operon via the LuxR transcriptional activator. The synthesis of AI-1 from bacterial metabolic intermediates is dependent on luxI. Recently, we found a second V. fischeri autoinducer molecule, N-octanoyl-L-homoserine lactone (AI-2), that in E. coli also...

Coral reefs perform a major role in regulating marine biodiversity and serve as hotspot for highly dynamic and diverse microbiomes as holobionts. Corals around Ishigaki, however, are at risk due to tremendous stressors including elevation of seawater temperature, eutrophication and so on. However, no information is currently available on how Vibrio diversity fluctuates spatially and temporally ...

Hybridization probes specific for the luxA genes of four groups of luminous bacteria were used to screen luminous isolates obtained from the Persian Gulf, near Al Khiran, Kuwait Nine of these isolates were identified as Vibrio harveyi, a commonly encountered planktonic isolate, while three others showed no hybridization to any of the four probes (V. harveyi, Vibrio fischeri, Photobacterium phos...

BACKGROUND AphA is the master quorum-sensing (QS) regulator operating at low cell density in vibrios. Molecular regulation of target genes by AphA has been characterized in Vibrio harveyi and V. cholerae, but it is still poorly understood in V. parahaemolyticus. METHODOLOGY/PRINCIPAL FINDINGS The AphA proteins are extremely conserved in V. parahaemolyticus, Vibrio sp. Ex25, Vibrio sp. EJY3, V...

Pathogenic Vibrio species cause diseases in diverse marine animals reared in aquaculture. Since their pathogenesis, persistence, and survival in marine environments are regulated by quorum sensing (QS), QS interference has attracted attention as a means to control these bacteria in aquatic settings. A few QS inhibitors of Vibrio species have been reported, but detailed molecular mechanisms are ...