The aim of this study was to investigate the pathogenic potential and antibiotic resistance 59 Escherichia coli isolates from ready-to-eat (RTE) street food (n = 31) drinking water 28) sold in city Maputo, Mozambique. were characterized by XbaI subtyping analysis via pulsed field gel electrophoresis. Multiplex PCRs performed targeting five virulence genes (stx, lt, st, astA, eae) three groups a...

An 81-year-old man was admitted to a primary care hospital due to bloody diarrhea. The findings of abdominal computed tomography indicated ischemic colitis, so conservative therapy was started. On the 4th hospital day, the patient was transferred to our hospital because of renal dysfunction. Physical examination showed clouding of consciousness and abdominal distention. Abdominal computed tomog...

In 1982-3, 35 children from the West Midlands developed the haemolytic-uraemic syndrome. This was a higher incidence than expected and included an epidemic localised to the Wolverhampton area in July 1983 which comprised 11 cases in two weeks. Twenty three children were treated with dialysis, of whom three died. Six patients developed chronic renal failure, four of them from Wolverhampton. Extr...

Tasks of the work were study the possible E.coli contamination in red deer meat, identify pathogenic strains from isolated E.coli, determine their incidence in red deer meat and determine the presence of VT1, VT2 and eaeA genes for the pathogenic E.coli. 8 (10%) samples were randomly selected from 80 analysed isolates of E.coli and PCR reaction was performed on them. PCR was done both on initia...

Many methods have been developed for the rapid and sensitive detection of Escherichia coli O157:H7 in milk and other foodstuffs. This study was designed to evaluate the application of RIDA®QUICK Verotoxin/O157 Combi kit, an immuno-chromatographic detection method, for the detection of this pathogen in raw milk. RIDA®QUICK kit showed appreciable results in detecting Escherichia coli O157:H7 in r...

Two Escherichia coli O-rough:K1:H7 strains producing verotoxin 1 that were isolated from stool samples of two travelers with diarrhea who consulted our clinic after trips to the Indian Subcontinent and Central America were characterized. Both strains were sorbitol negative, the same phenotype presented by E. coli O157:H7, but in contrast they were beta-glucuronidase positive. Low-frequency rest...

Enterohemorrhagic Escherichia coli, including the serotype O157:H7 that is most commonly identified with human disease, cause both sporadic cases and outbreaks of non-bloody diarrhea and hemorrhagic colitis. In about 10% of infected subjects, the hemolytic uremic syndrome (hemolytic anemic, thrombocytopenia, and acute renal failure) develops, likely as a consequence of systemic spread of bacter...