BACKGROUND Formation of struvite stones is associated with urinary tract infection by urease-producing bacteria. Biogenic crystal growth in natural and synthetic materials is regulated by the action of inhibitors, ranging from small ions, molecules to large macromolecules. MATERIALS AND METHODS We report the dynamics of in vitro crystallization of struvite in presence of vitamin C in syntheti...

Sir—In response to the letter of Drs. Frederiksen and Tønning [1], we note that ATCC 13361 is used as the standard strain for Pasteurella gallinarum identification. The biological features of the strains of ATCC 13361 and P. gallinarum isolated from our patient were the same. Both types of bacteria were gram-negative rods that were unable to grow on MacConky agar, and neither of them produced i...

Tightly spiral shaped Gram negative bacteria were seen in the gastric biopsies obtained from two patients undergoing gastroscopy. Active chronic gastritis was present in both patients and one patient also had gastric ulceration. Attempts to culture the organism by a number of methods were unsuccessful but positive urease results were obtained in both patients. Both patients were treated with co...

In this present study, we developed Brassica oleracea var. Capitata (red cabbage) extract (RCE) powder- incorporated colorimetric media for rapid, selective, sensitive detection of urease-postive bacteria, Klebsiella pneumoniae ATCC 54475 and Proteus mirabilis 25933. The major component RCE is anthocyanin groups which are in charge pH dependent color change. ureas test contains certain concetra...

Urease is the enzyme catalyzing the hydrolysis of urea into carbon dioxide and ammonia. This enzyme is substrate-specific, which means that the enzyme catalyzes the hydrolysis of urea only. This feature is a basic diagnostic criterion used in the determination of many bacteria species. Most of the methods utilized for detection of urease are based on analysis of its enzyme activity – the hydrol...

Campylobacter pylon or campylobacter like organisms (CLO) are curved bacilli which are found on the gastric mucosa of patients suffering from gastritis, peptic ulceration and other related diseases. These organisms were later renamed as helicobacter pylori. The diagnosis of HP is usually made on histology, culture, urease tests and serology. Although all these tests are 60-100% sensitive and sp...

Cryptococcus neoformans var. neoformans (74 isolates) and C. neoformans var. gattii (44 isolates) were used to test urease activity after growth on both yeast extract-glucose-peptone agar (YEPG) and on YEPG supplemented with 100 microM EDTA. Every isolate grown on YEPG agar for 48 h at 30 degrees C produced a positive reaction within 1 h in a modified rapid urease assay at 37 degrees C. However...

Bacterial urease, particularly from Proteus mirabilis, has been implicated as a contributing factor in the formation of urinary and kidney stones, obstruction of urinary catheters, and pyelonephritis. Weekly urine specimens (n = 1,135) from 32 patients, residing at two chronic-care facilities, with urinary catheters in place for greater than or equal to 30 days yielded 5,088 phenotypically and ...

objective: helicobacter pylori (h. pylori) is the most common pathogenic bacteria in the stomach. the aim of the current study was to explore the effect of oral garlic administration on bacterial urease activity inside the stomach and its contribution to the treatment of h. pylori infection.   materials and methods: in this clinical trial, 15 patients were studied quantitatively with urease bre...