In our endeavor to improve the nitrogen fixation efficiency of a soil diazotroph that would be unaffected by synthetic nitrogenous fertilizers, we have deleted a part of the negative regulatory gene nifL and constitutively expressed the positive regulatory gene nifA in the chromosome of Azotobacter chroococcum CBD15, a strain isolated from the local field soil. No antibiotic resistance gene or ...

The effects of the nitrogen sources sodium nitrate (NaNO3) and urea (CH4N2O) on growth, lipid production, and fatty acid composition of Nannochloropsis oceanica IMET1 were investigated. Nitrogen source affected cell density, dry cell weight, and lipid production. Cells grown in the nitrate medium increased dry cell weight and lipid weight in comparison with cells grown in the urea medium. The c...

1. The rate of transfer of plasma urea-nitrogen to rumen ammonia was measured by infusion of 15NH4Cl and [15N]urea into sheep given brome grass (Bromus inermis) or lucerne (Medicago sativa) pellets. Urea was infused into the rumen or abomasum of two sheep given brome grass in order to increase the concentration of rumen ammonia. 2. From 6.2 to 9.8 g/d of plasma urea-N were transferred to the ru...

In 1921 Nash and Benedict (11) advanced the hypothesis that urinary ammonia is formed in the kidney from some precursor in the blood. This conclusion was based upon the observation that the concentration of ammonia was higher in the renal vein than in the renal artery, that the quantity of ammonia in arterial blood was too small to supply the ammonia found in the urine, and that the arterial am...

An in vitro study was conducted using a 4 • 2 • 2 factorial design with RAC (readily available carbohydrate) levels of 30, 40, 50 and 60%, tallow levels of 0 and 3%, and urea levels of 0 and 2%. Acetic and propionic acid production and ammonia disappearance increased as the level of RAC increased from 30 to 50%. Tallow reduced acetic acid production and increased the amount of propionic acid pr...

Intracellular hydration may play a role in the regulation of protein and nitrogen metabolism. The hepatic removal of nitrogen by urea synthesis has a key regulatory role in nitrogen balance. The purpose of the present study was to establish the acute effects of dehydration on the hepatic kinetics of urea synthesis, quantified by functional hepatic nitrogen clearance (FHNC), in healthy volunteer...

BACKGROUND: Fertilizer management plays an important role to achieve sustainable agriculture. OBJECTIVES: Current study was conducted to assess response of agrophysiological traits of wheat to apply different level of fertilizer and biologic fertilizer (Phosphorus and Nitrogen). METHODS: This research was carried out via factorial e...

In sorbent-based hemodialysis, factors limiting a treatment session are urea conversion capacity and sodium release from the cartridge. In vitro experiments were performed to model typical treatment scenarios using various dialyzers and 4 types of SORB sorbent cartridges. The experiments were continued to the point of column saturation with ammonium. The urea nitrogen removed and amount of sodi...

Milk urea nitrogen (MUN) of dairy cows was determined in relation to breed. A total of forty (40) Pabna and red Chittagong dairy cattle each of twenty (20) were selected to know the milk composition, nutritional status and milk urea nitrogen. Live weight of Pabna Cattle (187±14 kg) was higher than that of red Chittagong cattle (174±4 kg). In Pabna cattle milk yield (4.94 kg), minerals (0.15%) a...

Glycine has been regarded as a poor source of nitrogen for total parenteral nutrition. Two prospective randomised cross over controlled clinical trials were undertaken to compare the efficacy of high and low glycine containing amino acid solutions in parenterally fed malnourished hypoalbuminaemic patients with gastrointestinal disease. In the first study (n = 9), amino acid solutions in which g...