The amount of 140 individual proteins of E. coli B/r was measured during balanced growth in five different media. The abundance of each protein was determined from its absolute amount in 14C-glucose-minimal medium and a measurement of its relative amount at each growth rate using a double labeling technique. Separation of the proteins was carried out by two-dimensional gel electrophoresis. This...

A new method for spot matching in two-dimensional gel electrophoresis images using a cumulative frequency matrix is proposed. The method improves on the weak points of the previous method called 'spot matching by topological patterns of neighbour spots'. It accumulates the frequencies of neighbour spot pairs produced through the entire matching process and determines spot pairs one by one in or...

A two-stage fiber-based femtosecond amplification system is presented, based on chirped-pulse amplification in highly nonlinear regime. The amount of self-phase modulation is separately adjusted in each stage selecting the proper stretching ratio in order to compensate gain narrowing. Analytical design rules are validated using numerical simulations. Our experimental implementation leads to the...

exact solution for steady two-dimensional flow of an incompressible magma is obtained. themagmatic flow is studied by considering the magma as a second grade fluid. the governing partialdifferential equations are transformed to ordinary differential equations by symmetry transformations. resultsare discussed through graphs to understand the rheology of the flowing magma

A subset W of the vertices of a graph G is a resolving set for G when for each pair of distinct vertices u,v in V (G) there exists w in W such that d(u,w)≠d(v,w). The cardinality of a minimum resolving set for G is the metric dimension of G. This concept has applications in many diverse areas including network discovery, robot navigation, image processing, combinatorial search and optimization....

Distributed circuits using transmission lines (T-lines) or inductors exhibit high gain-bandwidth-products (GBW), as required by broadband communication systems. In this paper, the design of non-uniform downsized distributed amplifiers (DAs) is explored. Conventional DAs employ a topology in which inductors or T-lines separate the gain stages, yet the output currents from individual stages combi...

Ever since its discovery, graphene bears great expectations in high frequency electronics due to its irreplaceably high carrier mobility. However, it has long been blamed for the weakness in generating gains, which seriously limits its pace of development. Distributed amplification, on the other hand, has successfully been used in conventional semiconductors to increase the amplifiers' gain-ban...