Inhibitors of trypsin and chymotrypsin from several highly inbred genetic types of lima beans were fractionated by ion exchange chromatography. Six chromatographically distinct inhibitors were found in all cases. However, the relative distribution of these components fell into two types, dependent upon the variety of bean used. One milligram of one of the chromatographically homogeneous fractio...

A trypsin inhibitor has been isolated in 54% yield from bovine pancreatic juice by gel filtration on Sephadex G-75 at pH 8.1 and by elution chromatography on DEAE-cellulose at pH 9.0. It appears to be homogeneous by equilibrium chromatography, equilibrium sedimentation ultracentrifugation, and amino acid analysis, and on the basis of the stoichiometry of its interaction with trypsin. The polype...

Soymilk is lower in calories compared to cow's milk, since it is derived from a plant source (no cholesterol) and is an excellent source of protein. Despite the beneficial factors, soymilk is considered as one of the most controversial foods in the world. It contains serine protease inhibitors which lower its nutritional value and digestibility. Processing techniques for the elimination of tryp...

This work aimed to develop a novel methodology based on aqueous micellar systems (AMS), for producing soy protein concentrates (SPC) from soybean flour and recovering high-valuable bioactive compounds as by-products. Ethoxylated aliphatic alcohols Tergitol 15-S-7 15-S-9, non-toxic biodegradable surfactants, were selected form the AMS. The consisted of an extractive stage with AMS, which rendere...

We have developed a screening method to identify stable protein mutants from a large number of sequences using a cellular quality control system. This method was used to screen amino acid pairs substituted for the disulfide (S-S) bond between residues 14 and 38 of bovine pancreatic trypsin inhibitor. The mutants selected could be divided into two groups: one with mutation C14G and the other wit...

A methodology for large scale molecular dynamics simulation of a solvated polarizable protein, using a combination of permanent and inducible point dipoles with fluctuating and fixed charges, is discussed and applied to the simulation of water solvated bovine pancreatic trypsin inhibitor (BPTI). The electrostatic forces are evaluated using a generalized form of the P3M Ewald method which includ...

Polymorphonuclear invasion of the wounded rat cornea is quantitatively described. The inflammatory cells enter the wounded tissue during the 5th postoperative hour. They steadily increase in number until they reach a maximum between 24 and 36 hours and return to normal by about the 6th day. Six hour wounds are used to evaluate the influence of topically applied drugs on the polymorphonuclear in...

We have used magnetic relaxation dispersion to study bovine pancreatic trypsin inhibitor (BPTI) self-association as a function of pH, salt type and concentration, and temperature. The magnetic relaxation dispersion method sensitively detects stable oligomers without being affected by other interactions. We find that BPTI decamers form cooperatively under a wide range of solution conditions with...

Conditions for the production of the trypsin inhibitor from Cephalosporium sp. KM 388 were investigated. Polypeptone-meat extract-glucose medium supported excellent production of the trypsin inhibitor. In this medium, polypeptone and meat extract were utilized both as carbon and nitrogen sources and as limiting substrates for the cell growth. Glucose was consumed during the stationary growth ph...