Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

Herpes simplex virus 1 (HSV-1) ICP27 and ICP8 proteins have both been implicated in the transcription of late genes and regulation of viral gene expression. We showed previously that ICP27 and ICP8 associate with the RNAP II holoenzyme (Zhou and Knipe, J. Virol. 76, 5893-5904). Here, we demonstrate that ICP27 and ICP8 coprecipitate from lysates of HSV-1-infected HEp2 cells and from lysates of i...

The alpha1- and beta1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purification of active recombinant enzyme on a nickel-affinity column. The enzyme has one heme p...

there has not yet accomplished a comprehensive research on the  details of  khāqānī's life incidents and his relationship with the courts of the kings and the lords ruled during his period, so a very vague image has remained of the faces of his praised ones. it would be undoubtedly impossible to get a minute understanding of the poems of this great poet of 12th century or even to try to co...

human as the superior of the creature was more important for pre-socrates, but by emerging socrates this remark become pale because he pointed to be average of human's powers. after many decades, some grand-phipspher such as kierkegaard, nietzsche, martin heidegger and jean-paul sartre entered and they have taken attention to human and established existentialism.this academy spoke about hu...

religious themes are dominant in the works of georges bernanos , the catholic writer . good and evil and the human condition have always been the main concerns of religious writers. these writers try to dramatize the confrontation of man with temptations. recurring topics in the writings of bernanos are ; a desire for innocence , seeking truth , fighting evil , divine grace , and the role of fa...