Statistical experimental design was used to optimize the conditions viz. Ethanol, Ammonium phosphate and Biotin concentration of L-Glutamic acid from Brevibacterium roseum NCIM 2238 by submerged fermentation. Maximum L-Glutamic acid concentration of 53.01621 g/l was obtained. The optimum conditions were found to be ethanol concentration of 5.3646 %v/v, ammonium phosphate of 0.7081 %w/v and biot...

This study was intended to isolate the following pathogenic bacteria; Yersinia enterocolitica, Listeria monocytogenes, Staphylococcus aureus and Salmonella species. Out of these four organisms, L. monocytogenes was not found in all samples. Pathogens were isolated from 31.4% of the 150 samples of open ice cream and 12.0% was isolated from packed ice cream. The targeted pathogens were not found ...

Chestnuts were collected either every 7 days from suspended nets used to intercept the fruits, every 2 days from the ground or every 7 days from the ground. Nuts were visually inspected after collection for the presence of exit holes of the chestnut weevil (Curculio elephas) and the chestnut moth (Cydia splendana), and 20 nuts per sampling and tree were bisected to assess fungal colonization. A...

Leptosphaeria korrae, the causal agent of necrotic ring spot, is a destructive patch disease of Kentucky bluegrass. To develop a rapid molecular test for the detection of this pathogen, an assay based on the polymerase chain reaction was developed utilizing the internal transcribed spacer region 1 (ITS 1) of L. korrae ribosomal DNA. DNA sequence comparison showed 94.8% similarity of the ITS 1 r...