Purpose: To determine whether mobile trabecular meshwork cell$ can contract collagen matrices in vitro Methods: Bovine and human meshwork cells were cultured and seeded into or onto type I collagen matrices in sterile conditions. The tloating matrices were measured on a daily basis to determine redtlction in diameter over time. Results: The meshwork cells contracted the collagen gells in a time...

PURPOSE To determine the genomic and proteomic expression changes in human trabecular meshwork cells when they are treated with transforming growth factor (TGF)-beta. METHODS Human trabecular meshwork cells from five donors were cultured for 3 days with 1 ng/mL of either TGF-beta1 or -beta2. Changes in gene expression determined with gene microarrays and alterations in protein expression dete...

The mechanisms of erythrocyte disposal by the trabecular meshwork in the human eye have not been extensively studied by electron microscopy. At the present time there is one short report on the ultrastructural changes induced by post-traumatic recurrent hyphaemas in two elderly patients. In both patients, Yamashita and Rosen ( I965) observed infiltration of the endothelial meshwork by phagocyti...

Scanning electron microscopic studies of the trabecular meshwork are presented and discussed which suggest that the trabecular endothelium is distinctly different from the corneal endothelium. The trabecular endothelial cells appear to be larger, with less prominent cell borders, and to have a thinner basement membrane than the corneal endothelial cells. The transition between the two types of ...

To understand the development of the trabecular meshwork of the eye, floating cellular aggregates (multicellular spheroids) were formed from human trabecular cells in a non-adherent environment of culture and incubated for up to one month. Dissociated trabecular cells formed multicellular spheroids within one day in the non-adherent environment, and apoptosis continued to occur in the spheroids...

Purpose: To determine whether mobile trabecular meshwork cell$ can contract collagen matrices in vitro Methods: Bovine and human meshwork cells were cultured and seeded into or onto type I collagen matrices in sterile conditions. The tloating matrices were measured on a daily basis to determine redtlction in diameter over time. Results: The meshwork cells contracted the collagen gells in a time...

Purpose: To determine whether mobile trabecular meshwork cell$ can contract collagen matrices in vitro Methods: Bovine and human meshwork cells were cultured and seeded into or onto type I collagen matrices in sterile conditions. The tloating matrices were measured on a daily basis to determine redtlction in diameter over time. Results: The meshwork cells contracted the collagen gells in a time...

s and letters 1. Johnson, D.H., Bourne, W.M., Campbell, R.J., 1981 Mar. Ultrastructure of Descemet’s membrane in Fuchs’ dystrophy. Investig. Ophthalmol. Vis. Sci. 20, 114 (abstract 1). 2. Allen, R.C., Johnson, D.H., Epstein, D.L., 1982. A long-term clinical trial of betaxolol in glaucoma patients. Ophthalmology 89, 191. 3. Johnson, D.H., Richardson, T.M., Epstein, D.L., 1983. Trabecular meshwor...

The distribution of 35S-sulfate and 3H-glucosamine in the angular region of the hamster was studied by light and electron microscopic autoradiography following intraperitoneal injection of these compounds to hamsters. Exposed silver grains of 35S-sulfate were concentrated in the trabecular meshwork, sclera, and cornea, and grains of 3H-glucosamine were localized in the trabecular region. The ra...

Glaucoma is a progressive optic neuropathy characterized by the loss of ganglion cells and their axons. A major risk factor for glaucomatous visual field loss is elevated intraocular pressure (IOP), and several studies have shown that lowering IOP reduces the risk of glaucomatous progression. Currently, an increasing number of researches involve Rho kinase inhibitors, which are a new pharmacolo...