Cantharis Q has a long history in both folk and traditional medicine being used as an aphrodisiac. It is a crude alcoholic extract of blister beetle Lytta vesicatoria commonly known as Spanish fly having cantharidin, a venomous substance present in it. The aim of the present study was to evaluate the clinical application of Cantharis Q by assessing its effect on the vital organ, liver, for a ...

It has been difficult to extract a good quality total RNA from the plant parts (such as seeds) which contain high levels of phenolic compounds, carbohydrates and other compounds that bind and/or co-precipitate with RNA. A simple, rapid and efficient method for isolating total RNA from polyphenols and polysaccharide rich plant tissues has been developed. Seeds of leguminosae family were chosen f...

the importance of extra cellular matrix (ecm) in development and function of different cells has been reported but little is known about its role in human endometrial epithelial cells. the aim of the present study was to examine effects of artificial ecm (matrigel) and progesterone on the function and morphology of human endometrial epithelial cells in vitro. methods: endometrial samples were r...

Background: In the present investigation radiation and cadmium induced biochemical changes in the kidney of Swiss albino mice have been studied. Materials and Methods: For this purpose, adult male Swiss albino mice (6-8 weeks old) were divided into four groups. Group I (sham-irradiated), Group II (treated with CdCl2 solution 20 ppm), Group III (irradiated with 1.25, 2.5 and 5.0 Gy gamma rays), ...

Background: The varicocele (VCL) impacts the testicular tissue both by enhancing tissue inflammation and by down-regulating the antioxidant status. On the other hand, in varicocele patient the critical role of Hsp70 in spermiogenesis is identified. Therefore present study was estimated to evaluate the protective effect of testosterone and vitamin E on VCL-decreased Hsp70 expression and on VCL-i...

Adenosine deaminases acting on RNA (ADARs) have double-stranded binding domains and a deaminase domain (DD). We used the MS2 system specific guide RNAs to direct ADAR1-DD target adenosines in mRNA encoding-enhanced green fluorescence protein. Using this transfected HEK-293 cells, we evaluated effects of changing length position efficiency conversion amber (TAG) ochre (TAA) stop codons tryptopha...

microRNAs are small regulatory RNAs that are currently emerging as new biomarkers for cancer and other diseases. In order for biomarkers to be useful in clinical settings, they should be accurately and reliably detected in clinical samples such as formalin fixed paraffin embedded (FFPE) sections and blood serum or plasma. These types of samples represent a challenge in terms of microRNA quantif...

There have been described a wide variety of methods for the preparation of total RNA from eucaryotic cells (1, 2). These methods are mainly designed for the isolation of RNA from relatively large amounts of cells. In a situation of screening a large number of cell clones, minimized cell cultivation effort and preparation time for total RNA isolation will be of great advantage. We developed a me...