background: engineering new bone tissue with cells and a synthetic extracellular matrix represents a new approach for the regeneration of mineralized tissues compared with the transplantation of bone (autografts or allografts). methods: in this study, to mimic the mineral and organic component of natural bone, hydroxapatite (ha) and gelatin (gel) composite scaffolds were prepared. the raw mater...

the transcriptional factor sox2 regulates the expression of some of the developmental genes, which are essential for the maintenance of pluripotency of stem cells. sox2 also expresses in the female gamete during folliculogenesis, but its role remains ambiguous. the aim of this study was to investigate the expression of sox2 in the mice ovarian tissue during different stage of estrous cycle. adu...

objective(s):diabetes plays an important role in the induction of the liver injury. grape seed proanthocyanidin (gsp) have a wide range of medicinal properties against oxidative stress. in this study we evaluated antioxidant effects of gsp on liver in streptozotocin-induced diabetic rats. materials and methods: thirty male sprague–dawley rats were divided into three groups: control, untreated d...

objective(s): gentamicin is used for the treatment of gram-negative bacterial infections. however, gentamicin administration is limited because of nephrotoxicity. the aim of the present study was to evaluate the protective effect of crocin against gentamicin-induced nephrotoxicity in rats. materials and methods: thirty two male wistar rats received gentamicin (100 mg/kg, ip), with or without cr...

in the paper, the contents of calcium (ca), magnesium (mg), zinc (zn), copper (cu), iron (fe),manganese (mn), chromium (cr), lead (pb), cadmium (cd), aluminium (al), and nickel (ni) were determinedin the meat of roe deers, wild boars, and hares coming from central region of poland. measurements werecarried out using atomic absorption spectrometry. the ranges of median values found for the conce...

to define efficacy of pre-operative chemotherapy in down staging of advanced non-round cell soft tissue sarcomas. from sep 2002 to dec 2005, 70 patients were treated by ifosfamid, mesna, cisplatin, adriamycin, mitomycin and subsequent surgery. postoperatively, patients received radiotherapy in cases of microscopically incomplete resection or local recurrence. the median age of the patients was ...

the purpose of this study was to examine the acute and sub-acute effects of diazinon onhistopathological parameters of liver tissue and blood level of hepatic enzymes of male commonfrog (rana ridibunda). frogs were assigned to 9 groups (n=8, meaning 8 frogs in each group):control and treatment groups with 30, 60, 90 and 120 μg/l diazinon in water for one day or oneweek. hepatic enzymes containi...

this study was carried out to investigate the effects of dietary putrescine (put) on broiler’s response fed low crude protein (cp) diets. a total of 192 male day old chicks were fed with four dietary treatments including two levels of put (0 and 0.03%) and two levels of cp (normal and low) with factorial combinations. weekly growth performance, nutrient digestibility and intestinal morphology (...

the reliability of gene expression profiling based technologies and methods to find transcriptional differences representative for the original samples is influenced by the quality of the extracted rna. hence, rna extraction is the first step to investigate the gene expression and its function. consequently, the quality of extracted rna is really significant. correspondingly, this research was ...