background and aims: prostate cancer is the second most common cancer in men worldwide in men. matrix metalloproteinase 2 (mmp2) has a role in the invasion and destruction of the basement membrane and the extra-cellular matrix and facilitating the process of tumor cell invasion. the present study was conducted to compare the expression of mmp2 gene in prostate cancer (pca) and benign prostatic ...

introduction: the protozoan parasite, cryptosporidium spp., widely spreads in both raw and drinking waters. it is the causative agents of waterborne diarrhea and gastroenteritis in the world. in the present study, a molecular assay was used for the detection and quantification of cryptosporidium oocysts in environmental water samples. materials and methods: thirty surface water samples were col...

background lung cancer is one of the most common causes of death worldwide. although smoking and environmental pollutants are the most important risk factors of lung cancer, the role of infectious causes should also be considered in the pathogenesis and progress of lung cancer. objectives this study examined the relationship between helicobacter pylori and lung cancer through serology, real-tim...

vacuolar h+-translocation pyrophosphatase (v-ppase) and na+/h+ antiporter (v-na+/h+) are important transporters in plant cells and have essential roles against abiotic stresses. in this study, the effects of salt stress were surveyed on the transcription of v-ppase pump and na+/h+ antiporter genes at early times of nacl treatment in roots of halophytic grass leptochloa fusca through the real ti...

Unlike classical end-point analysis PCR, real-time PCR provides the data required for quantification of the target nucleic acid. The results can be expressed in absolute terms by reference to external quantified standards or in relative terms compared to another target sequence present within the sample. Absolute quantification requires that the efficiency of the amplification reaction is the s...

We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over conta...