The Tbd_0210 gene of the chemolithotrophic bacterium Thiobacillus denitrificans is annotated to encode a 60.5 kDa bifunctional enzyme with ATP sulfurylase and APS kinase activity. This putative bifunctional enzyme was cloned, expressed and structurally characterized. The 2.95 A resolution X-ray crystal structure reported here revealed a hexameric assembly with D(3) symmetry. Each subunit contai...

Geochemical data indicate that protons released during pyrite (FeS2) oxidation are important drivers of mineral weathering in oxic and anoxic zones of many aquatic environments, including those beneath glaciers. Oxidation of FeS2 under oxic, circumneutral conditions proceeds through the metastable intermediate thiosulfate (S2O3 (2-)), which represents an electron donor capable of supporting mic...

the mouteh refractory pyrite concentrates at pulp densities of 1.5%, 3%, 4.5% and 6% were treated, using thiobacillus ferrooxidans dsm 581 and the same bacteria adapted on the mouteh pyritic concentrate. compared with a non-adapted culture, use of an adapted inoculum of t. ferrooxidans increased bioleaching rate of iron by a factor of 1.940, 2.011, 1.859 and 1.559 for pulp densities 1.5%, 3%, 4...

Ni2+, Fe2+ and Cu2+ were attractants and aspartate was an apparent repellent for Leptospirillum ferrooxidans, a behaviour opposite to that for Escherichia coli. Membranes from L. ferrooxidans contained proteins with a molecular mass in the range of 80 kDa which were methylated in vitro. Methylation was stimulated in the presence of a membrane-free extract from E. coli, showing the response patt...

The laboratory-scale pilot of constructed wetlands has been in operation for six months; (1) an unsaturated vertical flow wetland (UVF-CW), this system was used to represent the classic wetlands, (2) a saturated (SVF-CW), evaluate effects condition on nitrogen removal and composition microbial community. results showed that saturation positiveley influenced efficiencies nitrogen, aeverage rate ...

Uranium (U) contamination of the environment causes high risk to health, demanding for effective and sustainable remediation. Bioremediation via microbial reduction soluble U(VI) is generating fractions (>50%) insoluble non-crystalline U(IV) which, however, might be remobilized by sulfur-oxidizing bacteria. In this study, efficacy Acidithiobacillus (At.) ferrooxidans Thiobacillus (T.) de...

World-wide appreciated strategy for the management and treatment of high level and long lived radioactive waste is to dispose it in a deep and stable geological formation. Microbial community present in the host or buffer material may compromise the effective performance and safety of waste disposal system. In the Czech Republic, bentonite from Černý vrch locality is planned to be used as a buf...