Treatment of human promonocytic leukemic cell line U937 with mild hyperthermia in the temperature range of 40-43°C resulted in differentiation of these cells into monocyte/macrophage-like cells in a heat dose and time dependent manner. This process was accompanied by marked morphological, functional and proliferational changes. U937 cells which normally grow in supension in the logarithmic...

Jian-Pi-Yi-Shen (JPYS) decoction is a traditional Chinese herbal formula. The present study aimed to investigate the effect of JPYS drug-containing serum on proliferation and activity mouse osteoblasts. Sprague‒Dawley rats were fed decoction, calcium supplement, or normal saline for 6 weeks, was collected. Mouse osteoblasts treated with serum, supplement blank control serum. Cell assayed using ...

Polymorphonuclear neutrophils (PMN) from patients with chronic granulomatous disease of childhood have impaired bactericidal activity and are deficient in diphosphopyridine nucleotide, reduced form of, (NADH) oxidase. Since hydrocortisone had been shown to inhibit NADH oxidation, experiments were undertaken to determine the effect of hydrocortisone on several parameters of human PMN function. T...

The reduction of nitroblue tetrazolium by superoxide radicals generated from photo-reactive riboflavin has been in use for more than four decades to detect superoxide dismutase (SOD) on nondenaturing polyacrylamide gels. SOD research in medicine and biochemistry has warranted the development of multiple assay variants to overcome specific experimental constraints or to combine the SOD assay wit...

The human promyeloid cell line HL-60 differentiates toward monocytes or granulocytes when treated with 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) or dibutyryl cAMP, respectively. When nondifferentiated cells were incubated for 20 min with 2 mM H2O2 and 0.1 mM sodium orthovanadate to inhibit their protein-tyrosine-phosphatase activity (Heffetz, D., Bushkin, I., Dror, R., and Zick, Y. (1990) J. Biol....

in chronic myelogenous leukemia (cml), the mature granulocytes originate from a stem cell line harboring an abnormal chromosome, therefore it is possible that metabolic-functional abnormalities occur in the morphologically mature cells. in the present study, the phagocytic activity including intracellular killing, nitro blue tetrazolium (nbt) reduction, and phagocytosis were studied in 37 cml p...

Biofilms are at the root of many infections largely because they are much more antibiotic resistant than their planktonic counterparts. Antibiotics that target the biofilm phenotype are desperately needed, but there is still no standard method to assess biofilm drug susceptibility. Staphylococcus epidermidis ATCC 35984 biofilms treated with eight different approved antibiotics and five differen...

In chronic myelogenous leukemia (CML), the mature granulocytes originate from a stem cell line harboring an abnormal chromosome, therefore it is possible that metabolic-functional abnormalities occur in the morphologically mature cells. In the present study, the phagocytic activity including intracellular killing, nitro blue tetrazolium (NBT) reduction, and phagocytosis were studied in 37 CML p...

Background: Through combining two synthetic and natural polymers, scaffolds can be developed for tissue engineering and regenerative medicine purposes. Methods: In this work, carboxymethyl chitosan (CMC; 20%) was grafted to Polycaprolactone (PCL) nanofibers using the cold atmospheric plasma of helium. The PCL scaffolds were exposed to CAP, and functional groups were developed on the PCL surface...