A DNA genomic library constructed from Bacillus stearothermophilus, a gram-positive, facultative thermophilic aerobe that secretes a thermostable beta-mannanase, was screened for mannan hydrolytic activity. Recombinant beta-mannanase activity was detected on the basis of the clearing of halos around Escherichia coli colonies grown on a dye-labelled substrate, Remazol brilliant blue-locust bean ...

Pseudomurein endoisopeptidases cause lysis of the cell walls of methanogens by cleaving the isopeptide bond Ala-ε-Lys in the peptide chain of pseudomurein. PeiW and PeiP are two thermostable pseudomurein endoisopeptidases encoded by phage ΨM100 of Methanothermobacter wolfei and phages ΨM1 and ΨM2 of Methanothermobacter marburgensis, respectively. A continuous assay using synthetic peptide subst...

The thermostabilization of some enzymes by amidination with dimethylsuberimidate was surveyed. In the enzymes investigated, a moderate increase of thermostability in glucose oxidase and invertase, and prominent increase in lactate dehydrogenase, alcohol dehydrogenase and ascorbate oxidase were observed. The increase of thermostability by amidination was suggesting the synergism of the enhanceme...

An efficient design of in situ combustion depends on accurate kinetic modeling of the crude oil oxidation. The kinetic triplet of the oxidation reactions of a heavy oil sample was investigated. Once the kinetic triplet is known, the kinetic equation would be deconvolved. The crude oil sample was obtained from Kuh-E-Mond reservoir, located in the southwest of Iran. The samples were analyzed usin...

A Multi-objective optimization procedure has been developed to determine some kinetic parameters of free radical polymerization of vinyl acetate based on genetic algorithm. For this purpose, mathematical modeling of free radical polymerization of vinyl acetate is carried out first and then selected kinetic parameters are optimized by minimizing objective functions defined from comparing exp...

organophosphorus chemicals are compounds which have been used as pesticides and insecticides in agriculture. they’re also used as nervous agents and have raised many problems for human and environment. among the most important methods of decontamination from these compounds are biodegradation methods. using oph enzyme in degradation the mentioned compounds is seen as one of the desirable ways, ...

The DNA shuffling technique has been used to generate libraries of evolved enzymes in thermostability. We have shuffled two thermostable cytidine deaminases (CDAs) from Bacillus caldolyticus DSM405 (T53) and B. stearothermophilus IFO12550 (T101). The shuffled CDA library (SH1067 and SH1077 from the first round and SH2426 and SH2429 from the second round) showed various patterns in thermostabili...

the aim of this study was to determine time dependent effects of high intensity interval training (hiit) on oxygen kinetics in young males. 40 active students were randomly assigned to four groups: one session of hiit, two sessions of hiit, four sessions of hiit and control. both hiit and control groups performed the incremental test on an ergometer bicycle. two days after the pretest, training...

the present work is focused on the degradation of 4-chloro-2-nitrophenol in aqueous solution containing nano titanium dioxide, hydrogen peroxide and the combination of nano-tio2/h2o2. the effect of experimental parameters, such as the amount of nano- tio2 concentration of h2o2, initial concentration of 4-chloro-2-nitrophenol and ph on the reaction was investigated. experimental data obtained un...

The thermostability profile of seven different electrophoretic variants of human erythrocyte GOT found in 13 different, unrelated families from a racially heterogeneous population was examined. The five different slow-variant and the two different fast-variant classes could be grouped into four different thermostability classes which were termed unstable, less stable, normal and more stable tha...