The parE10(Ts) mutation, which renders Escherichia coli thermosensitive for growth by inactivation of the essential E. coli topoisomerase topo IV, is lethal at all temperatures when PriA, the main replication restart protein, is absent. This lethality is suppressed by the activation of a PriA-independent replication restart pathway (dnaC809 mutation). This result suggests that topo IV acts prio...

A novel drug delivery system, enabling an in situ, thermally triggered drug release is described, consisting of an injectable thermoresponsive chitosan hydrogel containing doxorubicin-loaded thermosensitive liposomes. The design, fabrication, characterization, and an assessment of in vitro bioactivity of this formulation is detailed. Combining on-demand drug delivery with in situ gelation resul...

The thermosensitive phenotype of strain CH31, a derivative of Corynebacterium ammoniagenes ATCC 6872, was allocated by cloning, sequencing, and genetic complementation to a single C-->T exchange in the nrdE (nucleotide reduction) gene at nucleotide 1301. Protein modeling indicates the impaired surface hydrophobicity of NrdE(Ts) due to the S434F transition.

A smart supercapacitor was fabricated by loading a thermosensitive polymer P(NIPAM-co-SPMA) onto the surface of NiAl-layered double hydroxide (LDH) nanowalls grown on a flexible Ni foil substrate, which displays temperature-triggered on-off ion channels for controlling the electrochemical behavior.

Background: Polymeric materials are widely known as versatile materials, particularly for biomedical applications. The bioavailability, low toxicity, solubility, and permeability of this material play an essential role in implants, such joint replacements, bone plates, ligaments, vascular grafts, intraocular lenses, heart valves, sutures, dental implants. These can be formed either biodegradabl...

Hydrogels, networks of cross-linked hydrophilic polymers, are an important class of soft materials with many of their properties similar to those of biological tissues. While these macroscopic materials have been intensively studied and exploited in various technological areas, their nanosized counterparts, the so-called nanogels, are currently attracting significant attention for their potenti...