Crown gall disease caused by Agrobacterium tumefaciens is considered as the main bacterial disease of stone fruit rootstocks in Tunisia against which only prophylactic control measures are available. Therefore, twelve rhizobacterial strains and two reference antagonists Agrobacterium rhizogenes K84 and K1026 were investigated for their efficacy against the causal agent of the disease in vitro, ...

Crops can be devastated by pathogenic strains of Agrobacterium tumefaciens that cause crown gall tumors. This devastation can be prevented by the nonpathogenic biocontrol agent A. radiobacter K84, which prevents disease by production of the "Trojan horse" toxin agrocin 84, which is specifically imported into tumorgenic A. tumefaciens strains to cause cell death. We demonstrate that this biocont...

The Ti plasmid sequences (T-DNA) from the octopine-producing crown gall tumor A6S/2 were isolated by molecular cloning, using the bacteriophage lambda vector Charon 4A. Analysis of the cloned DNA segments indicates that the Ti plasmid sequences are covalently joined to plant nuclear DNA. These data demonstrate that genetic recombination between a eukaryote and a prokaryote can occur as a natura...