We present an instrument for simultaneously measuring TEER in up to 80 perfused epithelial tubules on OrganoPlate. The sensitivity, speed and ease of use enables screening during formation, drug exposure inflammatory processes.

Alpha-melanocyte-stimulating hormone (α-MSH) is a potent anti-inflammatory peptide with cytoprotective effect in various tissues. The present investigation demonstrates the ability of α-MSH to interact with intestinal epithelial cell monolayers and mitigate inflammatory processes of the epithelial barrier. The protective effect of α-MSH was studied on Caco-2 human intestinal epithelial monolaye...

Macrolides including azithromycin (AZM) can improve clinical symptoms in asthma regardless of infection status. The mechanisms underlying these beneficial effects are yet to be elucidated. The aim of this study was to determine the effect of AZM on the airway epithelial barrier both in an in vitro model and in patients with asthma. Primary human bronchial epithelial cells (HBEC) were grown at a...

Human choriocarcinoma-derived BeWo cells express high levels of breast cancer resistance protein (BCRP/ABCG2) with no functional P-glycoprotein (P-gp) (ABCB1) activity, making them a potential model to study bidirectional ABCG2-mediated drug transport. However, the original BeWo clone (B24) available to researchers does not form confluent monolayers with tight junctions required by the model. O...

OBJECTIVE Effect of fingolimod in multiple sclerosis (MS) is thought to involve the prevention of lymphocyte egress from lymphoid tissues, thereby reducing autoaggressive lymphocyte infiltration into the central nervous system across blood-brain barrier (BBB). However, brain microvascular endothelial cells (BMECs) represent a possible additional target for fingolimod in MS patients by directly ...

AIM To investigate toll-like receptor 2 (TLR2) and TLR4 expression, following bifidobacteria and low-dose EPEC endotoxin treatment, and intestinal barrier function in rat intestinal epithelial cell18 (IEC18). METHODS Six experimental groups were established - normal control, EPEC, Bifidobacteria infantis (B. infantis), B. longum, B. bifidum, and B. youth groups. Optimal EPEC endotoxin concent...

BackgroundVersaCross is a novel radiofrequency transseptal solution that may improve the efficiency and workflow of puncture (TSP). The aim this study was to compare VersaCross system with mechanical needle systems during mitral transcatheter edge-to-edge repair (M-TEER) PASCAL device.MethodsThis single-center retrospective consecutive patients who underwent M-TEER PASCAL. Transseptal undertake...

Abstract Background Mitral regurgitation (MR) and tricuspid (TR) often occur simultaneously symptoms of biventricular heart failure can overlap. There is currently no consensus on the management combined MR TR. Purpose To evaluate impact TR echocardiographic functional outcome after M-TEER. Methods 740 patients underwent M-TEER for moderate-to-severe at our center from 2010 to 2021. Patients we...

Abstract Funding Acknowledgements Type of funding sources: None. Background Transcatheter edge-to-edge repair (TEER) has recently become a therapeutic option for patients with severe mitral regurgitation (MR) who are unsuitable surgery, in order to improve clinical outcomes. MR is determinant structural and functional changes all cardiac chambers. The left atrium (LA) the right ventricle (RV) p...

Abstract Funding Acknowledgements Type of funding sources: Private grant(s) and/or Sponsorship. Main source(s): Trial funded by Abbott Background As the treatment tricuspid regurgitation with transcatheter edge-to-edge repair (TEER) becomes more widespread, a better understanding anatomy is increasingly important to properly select patients for this therapy. The diversity anatomies in real-worl...