We developed a multiplexed real-time PCR assay using four sets of gene-specific oligonucleotide primers and four TaqMan probes labeled with four different fluorophores in a single reaction for detection of total and pathogenic Vibrio parahaemolyticus, including the pandemic O3:K6 serotype in oysters. V. parahaemolyticus has been associated with outbreaks of food-borne gastroenteritis caused by ...

Neurons in primary visual cortex are widely considered to be oriented filters or energy detectors that perform one-dimensional feature analysis. The main deviations from this picture are generally thought to include gain controls and modulatory influences. Here we investigate receptive field (RF) properties of single neurons with localized two-dimensional stimuli, the two-dimensional Hermite fu...

conclusions chlorine-stressed cells of v. parahaemolyticus were more susceptible to adverse conditions than the non-stressed ones. pathogenic and non-pathogenic strains showed the same survival characteristics under the adverse conditions. these results should be considered in the development of food preservation measures. results findings of the current study revealed that the normal cells of ...

Vibrio parahaemolyticus is one of the major seafood-borne gastroenteritis-causing bacteria, frequently associated with consumption of raw or inappropriately cooked seafood. Thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) are considered major virulence factors for the organism (7). V. parahaemolyticus can be classified into 13 O serotypes and 71 K serotypes (3). Although vari...

Presented here is the first report describing the detection of potentially diarrheal Vibrio parahaemolyticus strains isolated from cultured bivalves on the Mediterranean coast, providing data on the presence of both tdh- and trh-positive isolates. Potentially diarrheal V. parahaemolyticus strains were isolated from four species of bivalves collected from both bays of the Ebro delta, Spain.

This commentary brings further support for the trace deletion hypothesis from a new study of OVS and OSV sentences in Hebrew, which are active constructions that involve object movement but no change in morphology. The results show that the comprehension of these constructions in Broca’s aphasia is impaired, and the performance is at chance level, as predicted by the TDH.