BACKGROUND Human telomerase reverse transcriptase (hTERT) has become an ideal target for development of anticancer therapy. Small interfering RNAs (siRNAs) are very powerful reagents for gene silencing and show promise for cancer gene therapy. However, only a small number of siRNAs have been demonstrated to be effective. For gene therapy targeting hTERT, it is essential to develop a robust syst...

Supplementary Figure 1. Chromosomal map of the hox operon in Synechocystis sp. PCC 6803. Schematic showing integration of the eGFP cassette at the C-terminus of hoxF. OriT(RK2) in the disruption cassette stands for origin of transfer and allows for conjugation. The cassette also includes a 19bp and 20bp primer site called P1 and P2 respectively. These are identical in all disruption cassettes. ...

This Tutorial Account aims to be a useful educational resource which describes how automate fluorine-18 positron emission tomography (PET) radiochemistry using cassette-based automated radiosynthesis platforms.

Toxin-antitoxin systems (TAS) are abundant, diverse, horizontally mobile gene modules that encode powerful resistance mechanisms in prokaryotes. We use the comparative-genomic approach to predict a new TAS that consists of a two-gene cassette encoding uncharacterized HicA and HicB proteins. Numerous bacterial and archaeal genomes encode from one to eight HicAB modules which appear to be highly ...

The gene cassettes found in class 1 integrons are generally promoterless units composed by an open reading frame (ORF), a short 5’ untranslated region (UTR) and a 3’ recombination site ( ). Fused gene cassettes are generated attC by partial or total loss of the from the first cassette in an array, creating, in attC some cases, a fusion with the ORF from the next cassette. These structures are r...

27. L. Redmond, S.-R. Oh, C. Hicks, G. Weinmaster, A. Ghosh, Nature Neurosci. 3, 30 (2000). 28. To create the CREST antibody, We used PCR to amplify full-length crest cDNA from P0 mouse brain total RNA and cloned it into pET21 vector for expression of His-tagged CREST protein in bacteria. After isopropyl-D-thiogalactopyranoside induction, CREST protein was mainly collected in the inclusion body...

Recombinase mediated cassette exchange (RMCE) is a process in which site-specific recombinases exchange one gene cassette flanked by a pair of incompatible target sites for another cassette flanked by an identical pair of sites. Typically one cassette is present in the host genome, whereas the other gene cassette is introduced into the host cell by chemical or biological means. We show here tha...

BACKGROUND According to several treatment guidelines, epoprostenol is an important treatment option for pulmonary arterial hypertension. However, the pharmacokinetic characteristics and poor stability of epoprostenol at room temperature make its administration challenging. We therefore studied temperature fluctuations between the drug administration cassette and atmosphere to promote the safe u...